W1118 line

Manufactured by Bloomington Drosophila Stock Center

The W1118 line is a widely used Drosophila melanogaster genetic line. It serves as a standard wild-type control strain for a variety of experimental applications.

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Lab products found in correlation

Jazz mix drosophila food, by Thermo Fisher Scientific (1 mentions) Elav gal4c155, by Bloomington Drosophila Stock Center (1 mentions)

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W1118 (165 citations)

2 protocols using w1118 line

CRISPR-Based Drosophila CTPS Transgenic Lines

Cited 1 time

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CRISPR/Cas9 technology was used to establish the C-terminal mChe-4V5 tagged CTPS knock-in fly according to homology-directed repair procedures previously described by researchers at Fungene Biotech (Liu et al., 2022 (link); Bassett et al., 2013 (link); http://www.fgbiotech.com).
To generate transgenic UAS-CTPS and UAS-CTPS^MU flies, the cDNAs encoding Drosophila CTPS were produced by RT-PCR using the total RNAs extracted from the w¹¹¹⁸ line (#3605, from the Bloomington Drosophila Stock Center), as previously described by researchers at the Core Facility of Drosophila Resource and Technology, SIBCB, CAS (Liu et al., 2022 (link); Ni et al., 2008 (link)). The transgenic lines were backcrossed into the w¹¹¹⁸ background for over five generations before further genetic manipulations.

Liu J., Zhang Y., Wang Q.Q., Zhou Y, & Liu J.L. (2023). Fat body-specific reduction of CTPS alleviates HFD-induced obesity. eLife, 12, e85293.

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Drosophila Alzheimer's Disease Model

Cited 2 times

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All fly lines were raised under standard conditions at 20–25°C on standard yeasted Drosophila media (Applied Scientific Jazz Mix Drosophila Food; Thermo Fisher Scientific). The pan-neuronal driver elav-Gal4^C155 (#458) and the transgenic UAS line carrying human Aβ₄₂ (#33769) were purchased from Bloomington Drosophila Stock Center. We generated stocks carrying UAS-Tip60 (UAS-dTip60^WT C) previously (Lorbeck et al. 2011 (link)). The transgenic UAS-Tip60 fly line was crossed into the UAS-Aβ₄₂ background using standard genetic techniques, as previously described (Pirooznia et al. 2012 (link)), to generate the UAS-Aβ₄₂;UAS-Tip60 double transgenic fly line. The w¹¹¹⁸ line (Bloomington Drosophila Stock Center, #3605) served as the genetic background control. All experimental crosses were performed at an average physiological temperature of 20–25°C.

Zhang H., Karisetty B.C., Bhatnagar A., Armour E.M., Beaver M., Roach T.V., Mortazavi S., Mandloi S, & Elefant F. (2020). Tip60 protects against amyloid-β-induced transcriptomic alterations via different modes of action in early versus late stages of neurodegeneration. Molecular and cellular neurosciences, 109, 103570.

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