This study was approved by the Institutional Animal Care and Use Committee of Taipei Medical University (license no. LAC-2016-0295) and according to protocols approved by the Association for the Evaluation and Accreditation of Laboratory Animal Care. BALB/cByJNarl male mice aged 4 weeks were purchased from the National Laboratory Animal Center (Taipei, Taiwan) a week before the experiments began. Animals were housed in metal cages with hardwood chip bedding with a 12 h light-dark cycle and free access to laboratory food with a standard chow diet (Rodent Laboratory Chow no. 5001, Ralston Purina Company, St. Louis, MO, USA) and water. The facility temperature was maintained at 20–23°C and the relative humidity was between 36 and 57%; at the same time, minimal environmental stress and basic environmental enrichment were carried out in strict accordance with the recommendations of our institutional guidelines. Blood sugar levels of all mice were measured with a
One Touch II blood glucose meter (Lifescan, Milpitas, CA, USA) before beginning the experiments. At 6 h after the last feeding, the fasting blood sugar level was obtained from the tail vein of each animal. Blood sugar levels of the mice ranged from 110 ∼ 140 mg/dl.
Chen C.M., Houng J.Y., Ko T.L., Juan S.H, & Chou H.C. (2022). Sigesbeckia orientalis Extract Ameliorates the Experimental Diabetic Nephropathy by Downregulating the Inflammatory and Oxidative Stress Signaling Pathways. Evidence-based Complementary and Alternative Medicine : eCAM, 2022, 3323745.
