The antibodies applied in this study were described in Table S1 . CHX (Millipore, #508739) was purchased from Calbiochem (Germany). NH4Cl (Sigma, #A9434), 3-MA (Sigma, #M9281), CQ phosphate (Sigma, #PHR1258), MG-132 (Sigma, #C-2211), and Dimethyl sulfoxide (DMSO, Sigma, #D2650) were obtained from Sigma-Aldrich (Shanghai, China). All other reagents and solvents were commercially available, and were used as received unless otherwise noted.
Cq phosphate
Manufactured by Merck Group
Sourced in China, United States
CQ phosphate is a laboratory reagent used in various analytical and experimental procedures. It serves as a source of phosphate ions. The product's core function is to provide a controlled supply of phosphate to support specific chemical or biochemical reactions, without interpretation or extrapolation on its intended use.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
B27 supplement, by Thermo Fisher Scientific (2 mentions)
Mg132, by Merck Group (1 mentions)
Nh4cl, by Merck Group (1 mentions)
1640 medium, by Thermo Fisher Scientific (1 mentions)
Dispase, by Thermo Fisher Scientific (1 mentions)
Collagenase type 4, by Thermo Fisher Scientific (1 mentions)
Glucose free dmem, by Thermo Fisher Scientific (1 mentions)
Matrigel 354277, by Corning (1 mentions)
Dulbecco s modified eagle medium dmem f12, by Thermo Fisher Scientific (1 mentions)
B 27 supplement minus insulin, by Thermo Fisher Scientific (1 mentions)
H9 wa09, by WiCell (1 mentions)
Accutase, by Thermo Fisher Scientific (1 mentions)
Mitomycin c, by Merck Group (1 mentions)
Y 27632, by Merck Group (1 mentions)
Gelatin, by Merck Group (1 mentions)
Mtesr1, by STEMCELL (1 mentions)
2 protocols using cq phosphate
1
Antibody Validation and Chemical Reagents
2
Optimized Stem Cell Culture Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Dulbecco’s modified Eagle medium (DMEM)/F12, glucose-free DMEM, Roswell Park Memorial Institute (RPMI) 1640 medium, fetal bovine serum (FBS), penicillin/streptomycin, collagenase type IV, dispase, accutase, B27 supplement, B27 supplement minus insulin, and 0.05% trypsin-ethylenediaminetetraacetic acid (trypsin-EDTA) were purchased from Thermo Fisher Scientific (Waltham, MA, United States). The mTeSR1 and mTeSR1 5× supplements were purchased from STEMCELL Technologies Inc. (Vancouver, BC, Canada). Human embryonic stem cell line H9 (WA09) was procured from WiCell Research Institute (Madison, WI, United States). Matrigel (#354277) was supplied by Corning Life Sciences (Tewksbury, MA, United States). Mitomycin C, gelatin, Y27632, CQ phosphate, AZM, and all other unlisted reagents were acquired from Sigma-Aldrich (St. Louis, MO, United States).
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