Avance 400wb

The surface morphology of 2D PhenPtCl₂ nanosheets were examined with SEM (Hitachi-S4800). Optical images were taken by optical microscope (SOPTOP CX40M). XRD measurements were performed on a Bruker Dimension Icon D8 Advance system using Cu K_α radiation (40 kV, 40 mA). TGA data were collected by thermal analysis system (NETZSCH STA449 F3). Solid-state nuclear magnetic resonance was analyzed by superconducting (solid) nuclear magnetic resonance (Bruker AVANCE 400WB). Infrared spectra were gathered by FT-IR (Thermo Fisher Scientific nicolt Is50). Raman spectra were performed using a WITec alpha 300 R spectrometer with 532 nm laser excitation. The X ray photoelectron spectrometer (XPS) spectra of these samples were analyzed by ESCALAB 250Xi XPS equipped with a monochromatic Al K_α source (λ = 1486.6 eV). The adventitious C 1s peak of ~284.8 eV was used for charging corrections. HAADF-STEM images and energy dispersive X-ray spectroscopy (EDS) mapping were acquired by the FEI Titan Cubed Themis G2 300 with a probe corrector and a monochromator at 200 kV. Photoemission endstations BL11B in Shanghai Synchrotron Radiation Facility (SSRF) was used for the help in characterizations.

We conducted magnetic resonance imaging (MRI) of 21-month-old MAPT KI (Supplementary Fig. 2c), and AAV-CAPON/GFP-expressing mice 7 days and 3 months after AAV injection (Figs. 4a and 9b). The mice were anchored in the apparatus under anesthesia with 1.5% (v/v) isoflurane. During the scanning, the depth of anesthesia was monitored with a breathing sensor. Coronal/horizontal T2-weighted (T2W) MRI scans (2D TurboRAGE) of the whole brain were performed with a vertical-bore 9.4 T Bruker AVANCE 400WB imaging spectrometer with a 250 mTm⁻¹ actively shielded imaging gradient insert (Bruker BioSpin, Billerica, MA) controlled by Paravision software. T2W scans were performed with the following parameter settings: TR (repetition time) = 4342.2 ms, TE (echo time) = 53.8 ms, matrix dimensions = 256 × 256, flip angle = 180 degrees, field of view = 1.8 cm × 1.8 cm. We used a slice thickness of 0.5 mm and 29 slices with a scan time of 22 min 47 s to image the whole brain. Within the 29 scanned images, 8 images containing the hippocampal area were selected for further analysis. The hippocampal volume of each mouse was calculated using ImageJ software.

The solid-state ¹³C-NMR spectra were obtained on a Bruker Avance 400 WB (Bruker, Rheinstetten, Germany) utilizing a ¹³C resonant frequency of 100.61 MHz (magnetic field strength of 9.39 T). Approximately 100 mg of crystalline sample was packed into a zirconium rotor with a Kel-F cap. The cross‑polarization, magic angle spinning (CP-MAS) pulse sequence was used for spectral acquisition. Each sample was spun at a frequency of 8 kHz, and the magic angle setting was calibrated by the KBr method. Each data set was subjected to an 8 Hz line broadening factor and subsequently, Fourier transformed and phase corrected to produce a frequency domain spectrum. The chemical shifts were referenced to TMS using adamantane as an external secondary standard. The optimized recycle delay for T:HCl and T:OHN was 170 s and 50 s, respectively. The NMR spectra were processed with the ACD/SpecManager NMR program (version 10.0, Advanced Chemistry Development, Inc., Toronto, ON, Canada).