Histocore arcadia h

The cadaver was procured for gross anatomy training from the Anatomical Gift Program (Dayton, OH, USA). As a standard procedure, the cadavers used at the Gross Anatomy course are embalmed within 24 h of death in a formalin-based fixative solution. The thymus was harvested using 4.5″ surgical Sharp-Sharp scissors and thumb forceps (Nasco, Fort Atkinson, WI, USA), and we assessed the organ's weight, length, and thickness of each lobe, and the length of the transverse diameter. Subsequently, three samples from each thymic lobe were fixed and embedded in paraffin employing Histocore Arcadia-H (Leica Biosystems, IL, USA) and standard histology protocols (Gupta, 2011 ; Kim et al., 2016 (link)). Finally, paraffin-embedded tissue was cut into 5μM sections using HistocoreBiocut (Leica Biosystems, IL, USA) and placed on gelatin-coated microscope slides.

Six, 6- to 8-week-old female immunocompetent CBA/J mice were analyzed with hematoxylin and eosin (H&E) and Gram staining studies (The Jackson Laboratory; Bar Harbor, ME). The mice were each inoculated with ∼5 × 10⁷ CFU/mL E. coli in the right thigh. Inflammation in the left thigh of two mice in one group was induced by means of intramuscular administration of ∼5 × 10⁷ CFU/mL heat-inactivated E. coli. In another group of two mice, 750 μg/100 g of 10 mg/mL lipopolysaccharide (LPS) from E. coli O111:B4 (Sigma Aldrich; St. Louis, MO, USA) was intramuscularly administered. Two mice in the third group were treated with intramuscular administration of saline as a control. Myositis in mice was induced for 10 hours before left and right thigh tissue samples were harvested for staining and bacterial burden calculations. Tissue samples and microscope slides were prepared for H&E and Gram stains using the HistoCore Arcadia H (Leica, Wetzlar, Germany), and microscopic imaging was conducted on an Olympus BX50 L98-029 with an Olympus DP72 camera (Shinjuku, Tokyo, Japan) (Figure 6).