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Ab200836

Manufactured by Abcam
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Sourced in United Kingdom

Ab200836 is a primary antibody produced in rabbit. It is suitable for use in various immunoassay applications.

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Lab products found in correlation

Ab129002, by Abcam (1 mentions) Gtx213110 01, by GeneTex (1 mentions) Immobilon western chemiluminescent hrp substrate, by Merck Group (1 mentions) Immobilon western chemiluminescent hrp substrate kit, by Merck Group (1 mentions) Na934v, by GE Healthcare (1 mentions) Ab51013, by Abcam (1 mentions) Na931v, by GE Healthcare (1 mentions) Phenylmethylsulfonyl fluoride, by Merck Group (1 mentions) Complete protease inhibitor cocktail, by Roche (1 mentions) Sc 47778, by SCBT (1 mentions) Av40203, by Merck Group (1 mentions) Amersham ecl western blotting detection reagent, by GE Healthcare (1 mentions) Metronidazole, by Sangon (1 mentions) Neomycin, by Sangon (1 mentions) Resatorvid tak 242, by MedChemExpress (1 mentions) Alexafluor 647 rat anti mouse cd38 562769, by BD (1 mentions) Ampicillin, by Sangon (1 mentions)

4 protocols using ab200836

1

Protein Detection via Western Blotting

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Exogenously expressed proteins ANP32B and PB2, and the cellular control protein Vinculin were probed with primary antibodies rabbit anti-ANP32B (Abcam, ab200836; 1/2000), rabbit anti-influenza A virus PB2 (Genetex, GTX125925; 1/2000) and rabbit anti-Vinculin (Abcam, ab129002; 1/2000), respectively. The backsides of the primary antibodies were then detected with goat anti-rabbit secondary antibody conjugated to horseradish peroxidase (Genetex, GTX213110-01; 1/10,000). Detection was carried out using either Cytiva ECL Start Western Blotting Detection reagent (Amersham) or Immobilon Western chemiluminescent HRP substrate (Millipore).
Staller E., Carrique L., Swann O.C., Fan H., Keown J.R., Sheppard C.M., Barclay W.S., Grimes J.M, & Fodor E. (2024). Structures of H5N1 influenza polymerase with ANP32B reveal mechanisms of genome replication and host adaptation. Nature Communications, 15, 4123.
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2

Western Blot Analysis of Actin, FLAG, PHAP1, and PHAPI2

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For Western blot analysis, cells were lysed in NP-40 lysis buffer containing 1× cOmplete Protease Inhibitor Cocktail (Roche) and 1× Phenylmethylsulfonyl fluoride (Sigma-Aldrich) and cleared from the insoluble fraction by centrifugation at 17,000 × g for 5 min at 4°C. Samples were analyzed by SDS-PAGE and transferred onto nitrocellulose membranes. Membranes were probed with mouse monoclonal anti-Actin (Thermo Scientific, MS-1295), mouse monoclonal anti-FLAG M2 (Sigma-Aldrich, F1804), rabbit polyclonal anti-PHAP1 (Abcam, ab51013), and rabbit monoclonal anti-PHAPI2 (Abcam, ab200836) antibodies. Primary antibodies were detected using HRP-conjugated secondary anti-mouse (GE Healthcare, NA931V) and anti-rabbit (GE Healthcare, NA934V) antibodies and visualized using Immobilon Western Chemiluminescent HRP substrate kit (Millipore) according to the manufacturer’s instructions.
Nilsson-Payant B.E., tenOever B.R, & te Velthuis A.J. (2022). The Host Factor ANP32A Is Required for Influenza A Virus vRNA and cRNA Synthesis. Journal of Virology, 96(4), e02092-21.
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3

Immunoblotting of ANP32 Proteins

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huANP32A, huANP32B, chANP32A and their truncated versions were probed with rabbit anti-huANP32A (ab155148, Abcam), rabbit anti-huANP32B (ab200836, Abcam), and rabbit anti-chANP32A (AV40203, Sigma) antibodies. Beta-actin was probed with a mouse anti-beta-actin antibody (sc-47778, SCBT). Goat anti-rabbit and anti-mouse antibodies conjugated to horseradish peroxidase (HRP) were used as secondary antibodies. Detection was carried out using Amersham ECL Western blotting detection reagents (GE Healthcare).
Wang F., Sheppard C.M., Mistry B., Staller E., Barclay W.S., Grimes J.M., Fodor E, & Fan H. (2022). The C-terminal LCAR of host ANP32 proteins interacts with the influenza A virus nucleoprotein to promote the replication of the viral RNA genome. Nucleic Acids Research, 50(10), 5713-5725.
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4

Investigating Gut Barrier Integrity Modulators

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The antibodies of anti-BAFF (ab224710), anti-APRIL (ab200836) and anti-TLR4 (ab13556) were obtained from Abcam (UK). Occludin (CAT. no. SDH0158), zonula occludin 1 (ZO-1) (CAT. no. SDH0221) and Secretory immunoglobulin A sIgA; cat. no. SDH0166) were purchased from Shanghai SIMUWUBio Co., Ltd (Shanghai, China). The Cosmc Polyclonal antibody (19254-1-AP) was purchased from Proteintech. Nuclear factor-κB (NF-κB) p65 (D14E12) XP (8242) and MyD88 (D80F5) (4283) Rabbit mAb were purchased from Cell Signaling Technology (CST). Alexa Fluor 647 Rat Anti-Mouse CD38 (562 769), FITC Rat Anti-Mouse IgA (559 354) and PE Rat Anti-Mouse CD45R/B220 (553 089) were purchased from BD Pharmingen (USA). Ampicillin, neomycin and metronidazole were purchased from Sangon Biotech Co., Ltd (Shanghai, China). Vancomycin was obtained from VIANEX S.A. (PLANT C, Greece). A glycogen periodic acid-Schiff (PAS) staining kit was purchased from Beijing Solarbio Science & Technology Co., Ltd (Beijing, China). LPS was obtained from sigma (Code No L4391-1MG). TAK-242 (resatorvid) was purchased from MedChemExpress.
Zhu Y., He H., Sun W., Wu J., Xiao Y., Peng Y., Hu P., Jin M., Liu P., Zhang D., Wu J., Xie T., Huang L., He W., Wei M., Wang L., Xu X, & Tang Y. (2024). IgA nephropathy: gut microbiome regulates the production of hypoglycosilated IgA1via the TLR4 signaling pathway. Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association.
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