Glutest ace

Manufactured by Arkray

Sourced in Japan

The Glutest Ace is a laboratory instrument designed for the measurement of glucose concentrations. It provides accurate and reliable results, making it a suitable tool for various laboratory applications.

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Lab products found in correlation

Mouse insulin elisa kit, by Mercodia (2 mentions) Architect insulin, by Abbott (1 mentions) Lactate pro, by Arkray (1 mentions) Glucose cii test wako kit, by Fujifilm (1 mentions) Ultrasensitive mouse insulin elisa, by Mercodia (1 mentions)

5 protocols using glutest ace

Glucose, Insulin, and Lactate Dynamics

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BG was simultaneously measured in singlicate or duplicate using three different sets of automatic glucose analyzers (Glutest Ace; Arkray, Kyoto, Japan). The average value was calculated. BG0, BG90, BG105, BG120 and BG150 represent BG at –5, 90, 105, 120 and 150 min from the beginning of lunch, respectively. ΔBG90–105, ΔBG90–120 and ΔBG90–150 represent the differences in BG between BG90 and BG105, BG90 and BG120, and BG90 and BG150, respectively. Serum insulin level was measured using an immunoassay kit (Architect Insulin, Abbott Japan, Tokyo, Japan). INS90, INS120 and INS150 represent the serum insulin levels 90, 120 and 150 min from the beginning of lunch, respectively. ΔINS90–120 and ΔINS90–150 represent the difference in serum insulin level between INS90 and INS 120, and between INS90 and INS150, respectively. Plasma lactate level was determined in singlicate using an automated analyzer (Lactate Pro; Arkray). HbA1c level was assayed at Nagoya Clinical Center (Nagoya, Japan) using a latex-agglutination assay.

Takaishi T, & Hayashi T. (2017). Stair ascending–descending exercise accelerates the decrease in postprandial hyperglycemia more efficiently than bicycle exercise. BMJ Open Diabetes Research & Care, 5(1), e000428.

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Glucose and Insulin Measurement Protocol

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Blood glucose collected from the tail vein was measured using an auto analyzer (Glutest Ace, Arkray Inc., Kyoto, Japan). Plasma insulin concentration was measured using a Mouse Insulin Enzyme-Linked Immunosorbent Assay Kit (M1102, Morinaga Institute of Biological Science, Inc., Kanagawa, Japan). Portal plasma glucose concentration was measured using a Glucose CII Test Wako Kit (439–90,901, Fujifilm Wako Chemical Corporation, Osaka, Japan). Blood glucose and plasma insulin iAUC was calculated by summing the area of the increase from the pre-treatment value.

Matsunaga Y., Takahashi K., Takahashi Y, & Hatta H. (2021). Effects of glucose ingestion at different frequencies on glycogen recovery in mice during the early hours post exercise. Journal of the International Society of Sports Nutrition, 18, 69.

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Blood Glucose, Insulin, and FFA Assays

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Blood glucose concentrations were measured using an auto analyzer (Glutest Ace; Arkray Inc., Kyoto, Japan). Plasma insulin concentrations were measured using an enzyme-linked immunosorbent assay (ELISA) kit (Mouse Insulin ELISA Kit; Mercodia AB, Uppsala, Sweden). Plasma free fatty acid concentrations were measured using an assay kit (NEFA-C Wako; Wako, Tokyo, Japan).

Takahashi Y., Matsunaga Y., Banjo M., Takahashi K., Sato Y., Seike K., Nakano S, & Hatta H. (2019). Effects of Nutrient Intake Timing on Post-Exercise Glycogen Accumulation and Its Related Signaling Pathways in Mouse Skeletal Muscle. Nutrients, 11(11), 2555.

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Glucose and Insulin Quantification

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Blood glucose was measured using an auto analyzer (Glutest-Ace; Arkray Inc., Kyoto, Japan). Plasma insulin was measured using an ELISA kit (Ultrasensitive mouse insulin ELISA; Mercodia, Uppsala, Sweden).

Matsunaga Y., Sakata Y., Yago T., Nakamura H., Shimizu T, & Takeda Y. (2018). Effects of Glucose with Casein Peptide Supplementation on Post-Exercise Muscle Glycogen Resynthesis in C57BL/6J Mice. Nutrients, 10(6), 753.

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Blood Glucose and Insulin Measurements

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Blood glucose concentrations obtained from a tail vein were measured by an autoanalyzer (Glutest Ace; Arkray, Kyoto, Japan). The plasma insulin concentration was measured using an enzyme-linked immunosorbent assay (ELISA) kit (Mouse Insulin ELISA Kit; Mercodia, Uppsala, Sweden). We calculated the incremental area under the curve (iAUC) for the blood glucose concentration with the trapezoidal rule.

Takahashi Y., Matsunaga Y., Yoshida H., Shinya T., Sakaguchi R, & Hatta H. (2021). High Carbohydrate Diet Increased Glucose Transporter Protein Levels in Jejunum but Did Not Lead to Enhanced Post-Exercise Skeletal Muscle Glycogen Recovery. Nutrients, 13(7), 2140.

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