Log In Sign up
The largest database of trusted experimental protocols

Quantichrom gsh assay kit

Manufactured by BioAssay Systems
Visit Supplier
Sourced in United States

The QuantiChrom GSH assay kit is a colorimetric assay that quantifies the level of glutathione (GSH) in biological samples. The kit provides a simple, direct, and high-throughput method for measuring GSH concentration.

Automatically generated - may contain errors

Lab products found in correlation

Pierce protein assay kit, by Thermo Fisher Scientific (1 mentions) 4 hne assay kit, by Abcam (1 mentions) Mda assay kit, by Merck Group (1 mentions) Elisa max standard kit, by BioLegend (1 mentions) Il 10, by BioLegend (1 mentions) Tnf α, by BioAssay Systems (1 mentions) Tnf α, by BioLegend (1 mentions) Interleukin 6 (il 6), by BioLegend (1 mentions)

Spelling variants of this product

GSH assay kit (4 citations) QuantiChrom kits (2 citations)

5 protocols using quantichrom gsh assay kit

1

Quantifying Intracellular Glutathione Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols
Intracellular GSH concentrations were measured using the QuantiChrom GSH assay kit from BioAssay Systems (DIGT-250). The procedure was performed according to the manufacturer’s instructions. Briefly, 5,5′-dithiobis(2-nitrobenzoic acid) reacts with intracellular GSH to form a yellow product. Absorbance was measured at 412 nm, and GSH in cellular extracts was quantified via comparison to a calibration curve generated using GSH as a standard. Data were normalized to cellular protein content. Three independent experiments were performed for the calculation of means and SDs.
Anandhan A., Dodson M., Shakya A., Chen J., Liu P., Wei Y., Tan H., Wang Q., Jiang Z., Yang K., Garcia J.G., Chambers S.K., Chapman E., Ooi A., Yang-Hartwich Y., Stockwell B.R, & Zhang D.D. (2023). NRF2 controls iron homeostasis and ferroptosis through HERC2 and VAMP8. Science Advances, 9(5), eade9585.
+ Open protocol
+ Expand
2

Spinal Cord GSH Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Two-mm pieces of spinal cord centered on C5 level were homogenized in ice-cold PBS. Homogenates were centrifuged for 15 min at 10,000×g and the protein content of the supernatant was determined using the Pierce protein assay kit (Thermo Scientific, Bleiswijk, NL). The GSH content of the supernatant was analyzed using a QuantiChrom GSH Assay Kit according to the instructions of the manufacturer (BioAssay Systems, Hayward, USA).
Sprimont L., Janssen P., De Swert K., Van Bulck M., Rooman I., Gilloteaux J., Massie A, & Nicaise C. (2021). Cystine–glutamate antiporter deletion accelerates motor recovery and improves histological outcomes following spinal cord injury in mice. Scientific Reports, 11, 12227.
+ Open protocol
+ Expand
3

Ferroptosis Markers Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
The level of MDA, 4-HNE and GSH were measured to evaluate ferroptosis in different groups. Herein, the concentration of MDA, 4-HNE and GSH in tissue lysates were measured with the MDA Assay Kit (MAK085, Sigma-Aldrich), 4-HNE Assay Kit (ab238538, Abcam), and QuantiChrom™ GSH Assay Kit (DIGT250, Bioassay Systems) according to the manufacturer’s instructions respectively.
Liu P., Yuan J., Feng Y., Chen X., Wang G, & Zhao L. (2021). Ferroptosis contributes to isoflurane-induced neurotoxicity and learning and memory impairment. Cell Death Discovery, 7, 72.
+ Open protocol
+ Expand
4

Liver Function Assessment in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were weighed and blood samples were collected on day 28, the mice were euthanized via cervical dislocation and their livers were aseptically dissected out and weighed. Liver-to-body weight ratio: Liver weight/body weight. Each liver was homogenized, and the levels of cytokines [TNF-α (BioLegend, Inc.; cat. no. 430901); IL-6 (BioLegend, Inc.; cat. no. 431301) and IL-10 (BioLegend, Inc.; cat. no. 431411)], glutathione (GSH; BioAssay Systems; cat. no. DIGT-250), GSH peroxidase (GPx; BioAssay Systems; cat. no. EGPX-100) and TGs (Randox Laboratories Ltd.; cat. no. TR1697) were determined using the following commercially available kits: ELISA MAX™ Standard kit (BioLegend, Inc.; IL-6, cat. no. 431301; IL-10, cat. no. 431411; TNF-α, cat. no. 430901), QuantiChrom™ GSH assay kit (BioAssay Systems; cat. no. 75877-994), EnzyChrom™ GPx assay kit (BioAssay Systems; cat. no. 75878-136) and Randox TG assay kit (Randox Laboratories, Ltd.; cat. no. TR3823) according to the manufacturer's instructions.
Hsieh P.S., Chen C.W., Kuo Y.W, & Ho H.H. (2021). Lactobacillus spp. reduces ethanol-induced liver oxidative stress and inflammation in a mouse model of alcoholic steatohepatitis. Experimental and Therapeutic Medicine, 21(3), 188.
+ Open protocol
+ Expand
5

Quantification of Intracellular GSH

Check if the same lab product or an alternative is used in the 5 most similar protocols
Intracellular reduced GSH concentration was measured using the QuantiChrom GSH assay kit from BioAssay Systems. All the procedures were carried out according to the manufacturer’s instructions. All samples were carried out in triplicate for each experiment and the value from the untreated group was set as 1. The data represent the mean ± SD.
Shen T., Chen X.M., Harder B., Long M., Wang X.N., Lou H.X., Wondrak G.T., Ren D.M, & Zhang D.D. (2014). Plant Extracts of the Family Lauraceae: A Potential Resource for Chemopreventive Agents that Activate the Nuclear Factor-Erythroid 2-Related Factor 2/Antioxidant Response Element Pathway. Planta medica, 80(5), 426-434.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!