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Coolpix l110

Manufactured by Nikon
Sourced in Japan

The Coolpix L110 is a digital camera manufactured by Nikon. It features a 12.1-megapixel CCD sensor and a 15x optical zoom lens. The camera has a 3-inch LCD display and supports various shooting modes, including automatic and manual controls.

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5 protocols using coolpix l110

1

Estimating Archaeological Fish Size from Otolith Dimensions

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To estimate the size of archaeological fish, a linear regression between total length (TL, in mm) and otolith length (OL in mm) was constructed using 70 modern catfish otoliths (fish total length range: 240–837 mm). These otoliths belong to fish collected at the Río de la Plata Estuary (Argentina) between 2010 and 2015. The ventral face of each right archaeological and modern otolith was photographed using a Nikon Coolpix L110 digital camera at the same focal length. All images were taken with a black background with a 1 × 1 cm scale. Otolith length was measured in all pictures by using an image processing system (Image-Pro Plus 4.5).
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2

Electrochemical Characterization of Gold Microelectrodes

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All electrochemical measurements were carried out with a CH Instruments 1242B model potentiostat (Bee Cave, TX, USA) with an Au microwire working electrode. For FcTMA+ oxidation, and Ag/AgCl reference/counter electrode was used in a 2 electrode setup.9 (link) For stripping analysis, three Au electrodes were used as working, pseudo-reference and counter electrodes in a 3 electrode setup. Before use, fresh Au microwires were cut and cleaned by submerging in a solution containing 25% H2O2 and 50 mM KOH for 20 min, followed by submerging in ultra-pure water for 5 min.41 Ag/AgCl reference electrodes were fabricated by submerging an Ag microwire in 50 mM FeCl3 for 50 s then submerging in ultrapure water for 5 min.42 All videos and pictures were recorded with an Nikon Coolpix L110 camera and analyzed in MPC-HC (Windows). Conductive Ag paint was used to make an Ohmic contact between the microwire and the potentiostat’s alligator clips. Square-wave anodic stripping voltammetry was carried out through deposition at −1.6 V vs. an Au reference electrode for different times based on the device format. Square wave stripping voltammetry was carried out after a 10 s equilibration time, scanning from −1.5 to 0 V, with a 15 mV a step potential, 75 mV amplitude and 10 Hz frequency.
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3

Epidermal Cell Imaging and Analysis

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Videos and images of epidermal, NPCD stage cells, cells which do not undergo developmental PCD, were taken using DIC optics on a Nikon 90i (Nikon Canada, Mississauga, Ontario, Canada) microscope fitted with a DXM 1200c digital camera. Data acquisition was made using NIS Elements AR 3.10 software (Nikon Canada, Mississauga, Ontario, Canada). Digital photographs were taken with a Nikon Coolpix L110 camera (Nikon Canada, Mississauga, Ontario, Canada). Image edits were made with Adobe Photoshop and Adobe Illustrator (Adobe Systems Inc., San Jose, California, USA). Video editing was made with Adobe Premier Pro CS5 (Adobe Systems Inc., San Jose, California, USA). All additional file videos were standardized to 1 min duration regardless of original duration length and this standardization accounts for differing playback speeds. The time for cell death to occur is represented as the mean ± standard deviation and spans from the moment a given treatment was applied until tonoplast collapse or PM retraction were observed. Data are represented as the mean ± standard deviation.
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4

Thin-Layer Chromatography Analysis of N-Acetyl-Chitooligosaccharides

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Reaction products for various N-acetyl-chitooligosaccharides and colloidal chitin were analyzed using silica gel thin-layer chromatography (TLC). Aliquots (10 L) of reaction mixturesysis was carried according to the method of Tanaka et al. [22 (link)]. Aliquots (10 L) of reaction mixtures were spotted twice on TLC plates (TLC Silica gel 60 F254, Merck, Taipei, Taiwan) using micro-capillary (Drummond, Scientific, Broomall, Pennsylvania, USA), along with the respective (GlcNAG)1–3 standards. The plates were air dried. The mobile phase (the n-butanol:methanol:25% of ammonia:water ratio was 5:4:2:1) was allowed to run along the TLC plate until the solvent front reached more than 3/4 of its length, after which the plate was marked and dried. Then, the plates were developed by spraying with the reagent (4 mL of aniline, 4 g of diphenylamine, 200 mL of acetone, and 30 mL of 85% phosphoric acid) and heated at 180 °C for 3 min. Then, the TLC image was photographed using a Nikon Coolpix L110 digital camera (Nikon, Tokyo, Japan)
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5

Quantifying Kojic Acid Biosynthesis in Aspergillus flavus

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In order to validate the induction of kojic acid biosynthesis by increasing levels of H2O2, a modified method from Bentley66 was utilized. Briefly, A. flavus isolates were cultured on potato dextrose agar (PDA), PDA amended with 1 mM FeCl3, and PDA amended with 1 mM FeCl3 along with 15 mM H2O2. The presence of a bright orange coloration in the medium indicates the chelation of iron by kojic acid allowing for a qualitative comparison of kojic acid biosynthesis between the isolates.
The production of aflatoxin and kojic acid were also evaluated by thin layer chromatography according to methods used by Saruno et al.67 . Briefly, liquid culture medium was collected while harvesting mycelia for RNA isolation, spotted onto a silica gel 20 thin layer chromatography (TLC) plate, and dried at room temperature. The plate was then developed in butanol-acetic acid-water (4:1:5) and dried with warm air. Aflatoxin and kojic acid present within the culture medium samples were then visualized by UV fluorescence with excitation wavelengths of 365 and 254 nm, respectively, and photographed using a Nikon Coolpix L110 digital camera (Nikon, Tokyo, Japan).
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