Freeze-dried samples (1 g) were extracted in methanol (20 mL) with an Ultra Turrax T25 (IKA-Labortechnik, Staufen, Germany) at 1018 rpm until a uniform consistency was achieved. Samples were filtered (589 filter paper; Whatman, Germany) and appropriate aliquots of extracts were assayed by the Folin–Ciocalteu (FC) method [43 ] for total phenolic (TP) content, and by the ferric reducing antioxidant power (FRAP method) for antioxidant activity [44 (link)]. For HPLC analyses, extracts were further filtered with cellulose acetate syringe filters (0.45 μm porosity).
589 filter paper
Manufactured by Cytiva
Sourced in Germany
589 filter paper is a general-purpose filter paper designed for routine laboratory filtration applications. It is made from high-quality cellulose fibers and offers consistent performance in terms of flow rate and retention of particulates. The filter paper is available in various sizes to accommodate different laboratory needs.
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Lab products found in correlation
2 protocols using 589 filter paper
1
Quantifying Phenolic and Antioxidant Profiles
2
Phenolic and Antioxidant Analysis
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Freeze-dried (Scanvac, Coolsafe model, LaboGene, Denmark) samples (0.5 g) were extracted in methanol (20 mL) with an Ultra Turrax T25 (IKA-Labortechnik, Staufen, Germany) at 13,500 rpm until a uniform consistency was achieved. Samples were filtered (589 filter paper; Whatman, Germany) and appropriate aliquots of extracts were assayed by FC reagent for total phenolic (TP) content and by the ferric reducing antioxidant power (FRAP method) for antioxidant activity. For HPLC analyses, extracts were further filtered by cellulose acetate syringe filters (0.45 µm porosity).
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