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Anti frs2

Manufactured by Abcam
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Anti-FRS2 is a laboratory product used to detect the FRS2 protein. FRS2 is an adaptor protein that plays a role in signal transduction pathways. This product can be used in various research applications to study the expression and function of the FRS2 protein.

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Lab products found in correlation

Total mapk, by Cell Signaling Technology (1 mentions) P44 42 mapk erk1 2, by Cell Signaling Technology (1 mentions) Total mek1 2, by Cell Signaling Technology (1 mentions) Phospho mek1 2, by Cell Signaling Technology (1 mentions) Pi3 kinase p85 19h8, by Cell Signaling Technology (1 mentions) Phospho fgf receptor tyr653 654, by Cell Signaling Technology (1 mentions) Fgf receptor 2 d4l2v, by Cell Signaling Technology (1 mentions) Pfrs2, by Cell Signaling Technology (1 mentions) Phospho pi3 kinase p85 tyr458 p55 tyr199, by Cell Signaling Technology (1 mentions) Total akt, by Cell Signaling Technology (1 mentions) Phospho akt ser473, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Heparin sulfate, by STEMCELL (1 mentions) Vimentin, by Standard BioTools (1 mentions) Fibroblast growth factor 2 (fgf2), by Cell Signaling Technology (1 mentions) Phospho frs2, by Cell Signaling Technology (1 mentions) Pfrs2 y196, by Cell Signaling Technology (1 mentions) Cyclin d1, by Cell Signaling Technology (1 mentions) Phospho erk, by Cell Signaling Technology (1 mentions)

2 protocols using anti frs2

1

Western Blot Antibody Profiles

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Western blot assays were performed using established protocols with the following antibodies: phospho-Akt (Ser473) 1:1000 (Cell Signaling 9271), Total Akt 1:1000 (Cell Signaling 9272), phospho-MEK1/2 1:5000 (Cell Signaling 9154), Total MEK1/2 1:5000 (Cell Signaling 9122), phospho-FGF Receptor (Tyr653/654) 1:500 (Cell Signaling 3471), FGF Receptor 2 (D4L2V) 1:500 (Cell Signaling 23328), p44/42 MAPK (Erk1/2) 1:5000 (Cell Signaling 9101), Total MAPK 1:5000 (Cell Signaling 9102), pFRS2 1:1000 (Cell Signaling 3864), anti-FRS2 (abcam ab10425), phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) 1:1000 (Cell Signaling 4228), PI3 Kinase p85 (19H8) 1:000 (Cell Signaling 4257), β-actin 1:10000 (Cell Signaling 4967) and GAPDH 1:10000 (Santa Cruz sc-25778).
Krook M.A., Barker H., Chen H.Z., Reeser J.W., Wing M.R., Martin D., Smith A.M., Dao T., Bonneville R., Samorodnitsky E., Miya J., Freud A.G., Monk J.P., Clinton S.K, & Roychowdhury S. (2019). Characterization of a KLK2-FGFR2 fusion gene in two cases of metastatic prostate cancer. Prostate cancer and prostatic diseases, 22(4), 624-632.
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2

FGF2 and FGFR1 Signaling Pathway Assay

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Recombinant FGF2 was purchased from Gibco (#PHG0024). Heparin sulfate was purchased from StemCell Technologies (#07980). The following antibodies were used (all primary antibodies are rabbit derived unless otherwise noted): anti-FGFR1 (#9740), phospho-FGFR (Y653/654, #3476, mouse derived), phospho-FRS2 (Y436, #3861; Y196, #3864), PLCγ (#5690), phospho-PLCγ (Y783, #2821), ERK (#4695), phospho-ERK (T202/Y204 #4370), Akt (#4691), phospho-Akt (S473 #4060), STAT3 (#4904), phospho-STAT3 (Y705, #9145), E-cadherin (#3195), Vimentin (#5741), ZEB1 (#3396), N-cadherin (#13116), HIF1α (#14179), Bcl-XL (#2764), Hsp90 (#4877), FGF2 (#61977), TGFβ (#3711), Cyclin D1 (#2978), GAPDH (#2118), horseradish peroxidase (HRP)-linked rabbit IgG secondary antibody (#7074) and HRP-linked mouse IgG secondary antibody (#7076), from Cell Signaling Technology. Anti-FRS2 (#10425, mouse derived) was purchased from Abcam. AZD4547 was purchased from Selleck Chemistry. The following CyTOF antibodies were purchased from Fluidigm Inc.: STAT3 (#3173003A), pSTAT3 (#3158005A), Vimentin (#3154014A), TGFβ (#3163010B), SOX2 (#3150019B), and Nanog (#3169014A). Custom antibody-metal conjugations were performed using kits purchased from Fluidigm, Inc. (PRD002) and carrier-free antibodies targeting FGFR1 (#9740), pFGFR1 (#3476), pFRS2 Y196 (#3864), and ZEB1 (#3396) were purchased from Cell Signaling Technology.
Ryan M.R., Sohl C.D., Luo B, & Anderson K.S. (2018). The FGFR1 V561M Gatekeeper Mutation Drives AZD4547 Resistance through STAT3 Activation and EMT. Molecular cancer research : MCR, 17(2), 532-543.
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