7 hydroxycoumarin

Manufactured by Thermo Fisher Scientific

Sourced in United States

7-hydroxycoumarin is a chemical compound used as a fluorescent label and probe in biological research. It has excitation and emission wavelengths suitable for various analytical techniques. The core function of 7-hydroxycoumarin is to serve as a fluorescent marker or tracer in scientific investigations and experiments.

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Lab products found in correlation

Phenacetin, by Merck Group (3 mentions) Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (3 mentions) Bupropion hydrochloride, by Merck Group (2 mentions) Chlorzoxazone, by Cayman Chemical (2 mentions) Dextrorphan d3, by Merck Group (2 mentions) Acetaminophen d4, by Cayman Chemical (2 mentions) Hydroxybupropion d6, by Cambridge Isotopes (2 mentions) 7 hydroxycoumarin d5 sulfate, by LGC (2 mentions) 7 hydroxycoumarin 13c6 glucuronide, by LGC (2 mentions) Hydroxymidazolam 13c3, by Corning (2 mentions) Hydroxytestosterone d7, by Corning (2 mentions) Rat tail collagen 1, by Enzo Life Sciences (2 mentions) Sodium hydroxide (naoh), by Merck Group (2 mentions) Testosterone, by Cayman Chemical (2 mentions) Midazolam, by Cayman Chemical (2 mentions) Acetylacetone, by Tokyo Chemical Industry (1 mentions) Amitriptyline, by Cayman Chemical (1 mentions) 1 naphthol, by Merck Group (1 mentions) Fast blue rr salt, by Merck Group (1 mentions) Tau fluvalinate, by Merck Group (1 mentions)

Spelling variants of this product

4-chloromethyl-6,8-difluoro-7-hydroxycoumarin (CMF2HC, (4 citations) CellTracker Blue 4-chloromethyl-6,8-difluoro-7-hydroxycoumarin (CMF2HC; (3 citations) CellTracker Blue 4-chloromethyl-6.8-difluoro-7-hydroxycoumarin (2 citations)

5 protocols using 7 hydroxycoumarin

Compound sources and purification methods

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7-benzyloxyquinoline (BQ) was a product of CypEx (Dundee, UK). 7-hydroxycoumarin was obtained from Acros Organics, a part of Thermo Fisher Scientific (New Jersey, NJ). 7,8-benzoflavone (α-naphthoflavone, ANF) was manufactured by Indofine Chemical Company (Hillsborough, NJ). Amitriptyline and nortriptyline were the products of Cayman Chemicals (Ann Arbor, MI). Ivermectin was obtained from ApexBio (Boston, MA), and acetylacetone was the product of Tokyo Chemical Industry (Tokyo, Japan). Phenacetin was purchased from Sigma-Aldrich (St. Louis, MO). All other reagents were of ACS grade and used without additional purification.

Dangi B., Davydova N.Y., Maldonado M.A., Abbasi A., Vavilov N.E., Zgoda V.G, & Davydov D.R. (2020). Effects of Alcohol-Induced Increase in CYP2E1 Content in Human Liver Microsomes on the Activity and Cooperativity of CYP3A4. Archives of biochemistry and biophysics, 698, 108677.

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Analytical Reagents for Enzyme Assays

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Acetonitrile (99%), amitraz (> 98.0%), coumaphos (99.0%, Pestanal™ analytical standard), 7-ethoxycoumarin, tau-fluvalinate (98.7%), 1-naphthol (> 98.0%), 1-naphthyl acetate (> 98.0%), sodium dodecyl sulfate (~ 99%), Trizma base primary standard and buffer (> 99%), glutathione reductase from Baker's yeast, 1-chloro-2,4-dinitrobenzene (≥ 99%), and fast blue RR salt (95%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetone (HPLC grade), sodium phosphate dibasic anhydrous (98.0%), sodium phosphate monobasic monohydrate (99.5%), Triton X-100, hydrochloric acid (12.1 N), and sodium chloride (≥ 99.0%) were purchased from Fisher Scientific (Hampton, NH, USA). Coomassie brilliant blue G (electrophoresis grade) was purchased from MP Biomedical Supplies LLC (Solon, OH, USA). 7-hydroxycoumarin (99%) was purchased from Acros Organics (Pittsburg and Westchester, PA, USA).

Roth M., Wilson J., & Gross A.D. (2021). Assessing Varroa destructor acaricide resistance in Apis mellifera colonies of Virginia. Apidologie, 52(6), 1278-1290.

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Analytical Reagents for Drug Metabolism

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All chemicals and reagents were used as received unless otherwise specified. Chlorzoxazone, dextromethorphan (hydrobromide hydrate), (S)-mephenytoin, midazolam, testosterone, and acetaminophen-d₄ were purchased from Cayman Chemical Company. Collagen I (rat tail) was purchased from Enzo Life Sciences. Phenacetin, dextrorphan-d₃, bupropion hydrochloride, and sodium hydroxide (NaOH) were purchased from Millipore Sigma. Dimethyl sulfoxide (DMSO) and 7-hydroxycoumarin were purchased from Fisher Scientific. Hydroxybupropion-d₆ was purchased from Cambridge Isotope Laboratories. 6-hydroxyChlorzoxazone-d₂ was purchased from Clearsynth. 7-hydroxycoumarin-d₅-sulfate and 7-hydroxycoumarin-¹³C₆-glucuronide were purchased from Toronto Research Chemicals. 4-hydroxymephenytoin-d₃ was purchased from MuseChem. Hydroxymidazolam-¹³C₃ and hydroxytestosterone-d₇ were purchased from Corning.

Diprospero T.J., Brown L.G., Fachko T.D, & Lockett M.R. (2023). HepaRG cells undergo increased levels of post-differentiation patterning in physiologic conditions when maintained as 3D cultures in paper-based scaffolds. bioRxiv.

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Cytochrome P450 Enzyme Assay Protocol

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Coumarin, glucose-6-phosphate dehydrogenase, 7-methoxy-4-(trifluoromethyl)Coumarin (MFC), 7-hydroxy-4-(trifluoromethyl)Coumarin (HFC), magnesium chloride (MgCl₂), and nicotine adenine dinucleotide phosphate (NADP⁺) were purchased from Sigma Aldrich (St. Louis, MO). 3-Cyano-7-ethoxyCoumarin (CEC), 7-benzyloxy-4-(trifluoromethyl)Coumarin (BFC), and all recombinant microsomes from baculovirus-infected insect cells (supersomes) were purchased from BD Bioscience (Woburn, MA). 3-Cyano-7-hydroxyCoumarin (CHC) was purchased from Indofine Chemical Company. Potassium phosphate dibasic (K₂HPO₄) was obtained from Merck. Potassium phosphate monobasic (KH₂PO₄), D-glucose 6-phosphate sodium salt, 7-hydroxyCoumarin (HC), and dimethyl sulfoxide (DMSO) were purchased from Fisher Scientific Company. Vorozole and letrozole were provided by Brookhaven National Laboratory. All experiments were completed in all black, flat bottom Costar 96-well plates (Corning Incorporated, Corning, NY).

Raymond L., Rayani N., Polson G., Sikorski K., Lian A, & VanAlstine-Parris M.A. (2015). Determining the IC50 Values for Vorozole and Letrozole, on a Series of Human Liver Cytochrome P450s, to Help Determine the Binding Site of Vorozole in the Liver. Enzyme Research, 2015, 321820.

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Analytical Reagents for Drug Metabolism

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