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Prolong fade with dapi

Manufactured by Thermo Fisher Scientific
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Prolong Fade with Dapi is a mounting medium designed for fluorescence microscopy. It is used to preserve and protect fluorescent signals in fixed samples.

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Lab products found in correlation

Alexa fluorescein labeled secondary antibodies, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

ProLong Diamond Anti-fade reagent with DAPI (23 citations) Prolong Gold anti-fade medium with DAPI (10 citations) Prolong gold anti-fade mountant with DAPI (P36931) (3 citations) Prolong anti‐fade mounting media with DAPI (2 citations) ProLong Anti-Fade reagent with DAPI (41 citations) Prolong Gold anti-fade solution with DAPI (6 citations) PROLONG Anti-Fade Gold with DAPI (18 citations) Prolong with DAPI (40 citations) DAPI ProLong (2 citations)

2 protocols using prolong fade with dapi

1

Immunofluorescence Analysis of DNA Damage Markers

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Cells or OCT-embedded lungs on glass coverslips were washed in PBS, fixed in 4% paraformaldehyde, quenched with 50 mM glycine, permeabilized with 0.3% Triton X-100 in PBS, saturated with 3% goat serum (Life Technologies, Carlsbad CA, USA), and incubated with 53bp1 and γH2AX primary antibodies at room temperature (Novus Biologicals, Littleton CO, USA) for 1 hour, followed by incubation with Alexa fluorescein-labeled secondary antibodies (Life Technologies) for 45 minutes and mounted using Prolong Fade with Dapi (Life Technologies).
Demaria M., O’Leary M.N., Chang J., Shao L., Liu S., Alimirah F., Koenig K., Le C., Mitin N., Deal A.M., Alston S., Academia E.C., Kilmarx S., Valdovinos A., Wang B., de Bruin A., Kennedy B.K., Melov S., Zhou D., Sharpless N.E., Muss H, & Campisi J. (2016). Cellular senescence promotes adverse effects of chemotherapy and cancer relapse. Cancer discovery, 7(2), 165-176.
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2

mRFP Immunofluorescence Imaging

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Cells on glass coverslips were washed in PBS, fixed in 4% paraformaldehyde, quenched with 50 mM glycine, permeabilized with 0.3% Triton X-100 in PBS, saturated with 3% goat serum (Life Technologies), and incubated with mRFP primary antibody (Allele Biotechnology, #5F8) at room temperature for 1 hour, followed by incubation with Alexa fluorescein-labeled secondary antibodies (Life Technologies) for 45 minutes and mounted using Prolong Fade with Dapi (Life Technologies).
Kohli J., Campisi J, & Demaria M. (2017). A novel suicide gene therapy for the treatment of p16Ink4a-overexpressing tumors. Oncotarget, 9(7), 7274-7281.
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