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Flo min107

Manufactured by Oxford Nanopore
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The FLO-MIN107 is a nanopore sequencing device produced by Oxford Nanopore. It is a compact, portable, and low-power instrument designed for DNA and RNA sequencing. The FLO-MIN107 utilizes nanopore technology to detect and analyze the electrical signals generated as molecules pass through the nanopore, enabling real-time and direct sequencing without the need for amplification or labeling.

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Lab products found in correlation

Mnt 001, by Oxford Nanopore (1 mentions) Guppy v2, by Oxford Nanopore (1 mentions) Minit, by Oxford Nanopore (1 mentions) Rapid barcoding sequencing kit, by Oxford Nanopore (1 mentions) Minion, by Oxford Nanopore (1 mentions) Rneasy mini kit, by Qiagen (1 mentions)

2 protocols using flo min107

1

Rapid Nanopore Sequencing Library Prep

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Library preparation was carried out using the Rapid Barcoding Sequencing Kit (SQK-RBK004, Oxford Nanopore Technologies, Oxford, UK) according to the manufacturer’s instructions. The finished library was loaded onto a R9.5.1 MinION flow cell (FLO-MIN107, Oxford Nanopore Technologies). Sequencing was conducted under the standard settings using a MinION (Mk1B, Oxford Nanopore Technologies) in combination with a MinIT (MNT-001, Oxford Nanopore Technologies). Signal information (fast5) was basecalled with MinIT and Guppy v2.1.3 (Oxford Nanopore Technologies).
Forth J.H., Forth L.F., King J., Groza O., Hübner A., Olesen A.S., Höper D., Dixon L.K., Netherton C.L., Rasmussen T.B., Blome S., Pohlmann A, & Beer M. (2019). A Deep-Sequencing Workflow for the Fast and Efficient Generation of High-Quality African Swine Fever Virus Whole-Genome Sequences. Viruses, 11(9), 846.
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2

Full-length Transcriptome Analysis using MinION

Cited 1 time
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Total RNA was isolated and purified using an RNeasy Mini Kit (Qiagen). Library preparation for full-length transcriptome analysis using MinION (Oxford Nanopore Technologies) was performed as previously described [32 (link)]. In brief, 1.5 μg of the full-length-cDNA library was applied for 1D2 sequencing according to the manufacturer’s protocol with some modifications using the 1D2 sequencing kit, SQK-LSK308, and R9.5 flow cell, FLO-MIN107 (Oxford Nanopore Technologies).
Oka M., Xu L., Suzuki T., Yoshikawa T., Sakamoto H., Uemura H., Yoshizawa A.C., Suzuki Y., Nakatsura T., Ishihama Y., Suzuki A, & Seki M. (2021). Aberrant splicing isoforms detected by full-length transcriptome sequencing as transcripts of potential neoantigens in non-small cell lung cancer. Genome Biology, 22, 9.
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