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Integrin β1 sirna

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Integrin β1 siRNA is a laboratory tool designed to temporarily silence the expression of the integrin β1 gene. It functions by interfering with the translation of the integrin β1 mRNA, reducing the production of the integrin β1 protein.

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (3 mentions) Integrin β1, by Cell Signaling Technology (2 mentions) Integrin α5, by Cell Signaling Technology (2 mentions) Opti mem, by Thermo Fisher Scientific (2 mentions) Integrin α5 sirna, by Santa Cruz Biotechnology (2 mentions) Fibronectin pool, by Santa Cruz Biotechnology (2 mentions) Integrin β1 sirna b, by Santa Cruz Biotechnology (2 mentions) Fibronectin sirna, by Santa Cruz Biotechnology (2 mentions) Scrambled sirna, by Santa Cruz Biotechnology (1 mentions) Microliter 701, by Hamilton Company (1 mentions) Control sirna, by Santa Cruz Biotechnology (1 mentions) Control sirna sc 37007, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

SiRNAs (95 citations) Integrin β1 (24 citations) Integrin β1 siRNA B (2 citations)

5 protocols using integrin β1 sirna

1

Integrin and Fibronectin Knockdown Assay

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Cells were plated and left to grow overnight in 5% FBS/RPMI. 5%FBS/RPMI media was replaced with Opti-MEM (Invitrogen). Fibronectin pool, Fibronectin siRNA A GACUGGUGGUUACAUGUUAtt, Fibronectin siRNA B CGCAUCACUUGCACUUCUAtt, Fibronectin siRNA C GAUCCUGUCUACUUCACAAtt, Integrin α5 siRNA A GUCAGAAUUUCGAGACAAAtt, Integrin α5 siRNA B CCACUGACCAGAACUAGAAtt, Integrin β1 siRNA A GAGAUGAGGUUCAAUUUGAtt, Integrin β1 siRNA B GAUGAGGUUCAAUUUGAAAtt, (25nM, Santa Cruz), Integrin α5, Integrin β1, and PTEN (all 25nM, Cell Signaling Technologies), and BRAF (25nM, Thermo Scientific) siRNAs in complex with Lipofectamine 2000 (Invitrogen) were added. Scrambled, non-targeting siRNAs were used as controls. A final concentration of 5% FBS in RPMI was added the next day. For fibronectin knockdowns, final concentration 1% FBS in RPMI was added next day. Cells were transfected for 24-72 hours prior to treatment.
Fedorenko I.V., Abel E.V., Koomen J.M., Fang B., Wood E.R., Chen Y.A., Fisher K.J., Iyengar S., Dahlman K.B., Wargo J.A., Flaherty K.T., Sosman J.A., Sondak V.K., Messina J.L., Gibney G.T, & Smalley K.S. (2015). Fibronectin induction abrogates the BRAF inhibitor response of BRAF V600E/PTEN-null melanoma cells. Oncogene, 35(10), 1225-1235.
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2

Intracerebroventricular Drug Administration in Rats

Cited 1 time
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Intracerebroventricular (i.c.v) drug administration was performed as reported previously (Ma et al., 2017 (link); Tong et al., 2017 (link)). Briefly, rats were positioned prone in a stereotaxic apparatus under 2.5% isoflurane anesthesia. A burr hole was drilled into the skull according to the following coordinates relative to bregma: 0.92 mm posterior and 1.5 mm lateral. The needle of a 10 μL Hamilton syringe (Microliter 701; Hamilton Company, Reno, NV) was inserted into the left lateral ventricle through the burr hole 3.3 mm below the horizontal plane of bregma. Integrin β1 siRNA (10 μM/10 μL, Santa Cruz, USA) or scrambled siRNA (10 μM/10 μL, Santa Cruz, USA) was infused at the same rate 48 h before ICH induction.
Gong L., Manaenko A., Fan R., Huang L., Enkhjargal B., McBride D., Ding Y., Tang J., Xiao X, & Zhang J.H. (2018). Osteopontin attenuates inflammation via JAK2/STAT1 pathway in hyperglycemic rats after intracerebral hemorrhage. Neuropharmacology, 138, 160-169.
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3

Gene Manipulation for Cell Line

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GLUT1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (CA, USA; catalog number: sc-35493), integrin β1 siRNA and control siRNA were purchased from Santa Cruz Biotechnology (CA, USA; catalog number: sc-35674), and GLUT1 plasmid were purchased from OriGene (Rockville, USA; catalog number: SC116011). They were transiently transfected to cell line with Lipofectamine 2000 (Invitrogen, Carlsbad, USA; catalog number: 11668030) according to the instruction of manufacturer.
Zhao H., Sun J., Shao J., Zou Z., Qiu X., Wang E, & Wu G. (2019). Glucose Transporter 1 Promotes the Malignant Phenotype of Non-Small Cell Lung Cancer through Integrin β1/Src/FAK Signaling. Journal of Cancer, 10(20), 4989-4997.
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4

Silencing MLC20, Integrin β1, and MYH11

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For MLC20 and integrin β1 KD, control siRNA (SC-37007), MLC20 siRNA (SC-45414), and integrin β1 siRNA (SC-35674) were purchased from Santa Cruz Biotechnology. HASM cells were transfected with siRNA according to the manual of the manufacturer (Santa Cruz Biotechnology, Dallas, TX, USA). For MYH11 KD, control construct (sc-418922) and MYH11 CRISPR/Casp KO plasmids (sc-400695) were purchased from Santa Cruz Biotechnology. The experiments were performed according to the manual of the manufacturer.
Wang R., Arbel E, & Tang D.D. (2022). Smooth Muscle Myosin Localizes at the Leading Edge and Regulates the Redistribution of Actin-regulatory Proteins during Migration. Cells, 11(15), 2334.
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5

Integrin and Fibronectin Knockdown Assay

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Cells were plated and left to grow overnight in 5% FBS/RPMI. 5%FBS/RPMI media was replaced with Opti-MEM (Invitrogen). Fibronectin pool, Fibronectin siRNA A GACUGGUGGUUACAUGUUAtt, Fibronectin siRNA B CGCAUCACUUGCACUUCUAtt, Fibronectin siRNA C GAUCCUGUCUACUUCACAAtt, Integrin α5 siRNA A GUCAGAAUUUCGAGACAAAtt, Integrin α5 siRNA B CCACUGACCAGAACUAGAAtt, Integrin β1 siRNA A GAGAUGAGGUUCAAUUUGAtt, Integrin β1 siRNA B GAUGAGGUUCAAUUUGAAAtt, (25nM, Santa Cruz), Integrin α5, Integrin β1, and PTEN (all 25nM, Cell Signaling Technologies), and BRAF (25nM, Thermo Scientific) siRNAs in complex with Lipofectamine 2000 (Invitrogen) were added. Scrambled, non-targeting siRNAs were used as controls. A final concentration of 5% FBS in RPMI was added the next day. For fibronectin knockdowns, final concentration 1% FBS in RPMI was added next day. Cells were transfected for 24-72 hours prior to treatment.
Fedorenko I.V., Abel E.V., Koomen J.M., Fang B., Wood E.R., Chen Y.A., Fisher K.J., Iyengar S., Dahlman K.B., Wargo J.A., Flaherty K.T., Sosman J.A., Sondak V.K., Messina J.L., Gibney G.T, & Smalley K.S. (2015). Fibronectin induction abrogates the BRAF inhibitor response of BRAF V600E/PTEN-null melanoma cells. Oncogene, 35(10), 1225-1235.
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