Tritc conjugated antibody

Manufactured by Merck Group

TRITC-conjugated antibody is a fluorescent-labeled antibody used for various biomedical research applications. The antibody is conjugated with the Tetramethylrhodamine (TRITC) fluorescent dye, which emits light in the orange-red spectrum when excited by an appropriate light source. This product can be used for techniques such as immunofluorescence, flow cytometry, and cell imaging to detect and visualize target proteins or cells.

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Lab products found in correlation

Fibronectin, by Santa Cruz Biotechnology (1 mentions) Snail1, by Santa Cruz Biotechnology (1 mentions) Alexafluor 488 conjugated antibody, by Merck Group (1 mentions) E cadherin, by Santa Cruz Biotechnology (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Confocal laser microscope lsm 510 meta nlo, by Zeiss (1 mentions)

Close spelling variants of this product

TRITC-conjugated goat anti-mouse IgG secondary antibody TRITC-conjugated secondary antibody

2 protocols using tritc conjugated antibody

Antibody Sourcing for Cellular Analysis

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Primary antibodies to E-cadherin, GAPDH, fibronectin, SNAIL-1 and TBP were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA); primary antibody to β-catenin was from Abcam (Cambridge, UK); primary antibody to vimentin was from Sigma; primary antibody to α-SMA was from GeneTex (Irving, CA). Secondary horseradish peroxidase-conjugated anti-rabbit or anti-mouse antibodies were from goat (Bio-Rad Laboratories); AlexaFluor 488-conjugated antibody was from Millipore (Billerica, MA); TRITC-conjugated antibody was from Sigma.

Polimeni M., Gulino G.R., Gazzano E., Kopecka J., Marucco A., Fenoglio I., Cesano F., Campagnolo L., Magrini A., Pietroiusti A., Ghigo D, & Aldieri E. (2016). Multi-walled carbon nanotubes directly induce epithelial-mesenchymal transition in human bronchial epithelial cells via the TGF-β-mediated Akt/GSK-3β/SNAIL-1 signalling pathway. Particle and Fibre Toxicology, 13, 27.

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Multiparameter Immunofluorescence Imaging

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Cells were fixed with 4% paraformaldehyde in PBS for 15 min, permeabilized with 1% Saponin for 10 min, blocked with 1% BSA in PBS for 30 min and incubated with indicated antibodies for overnight, then incubated with tetramethylrhodamine isothiocyanate (TRITC)-conjugated antibody and fluorescein isothiocyanate (FITC)-conjugated antibody (Sigma-Aldrich) for 1 h in the dark. Nuclei were stained with 1 microgram/ml of 4′, 6-diamidino-2-phenylindole (DAPI) for 3 min. Pictures were taken using a Zeiss Confocal Laser Microscope LSM 510 META NLO (The Wayne State University Microscopy and Imaging Core Facility).

Harazono Y., Kho D.H., Balan V., Nakajima K., Hogan V, & Raz A. (2015). Extracellular galectin-3 programs multidrug resistance through Na+/K+-ATPase and P-glycoprotein signaling. Oncotarget, 6(23), 19592-19604.

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