Synthetic sequence scrambled sirna si nc

For transient knockdown experiments, the small-interfering RNAs (siRNAs) were as follows: FEZF1-AS1 siRNA (si-FEZF1-AS1), ZNF312B siRNA (si-ZNF312B) and synthetic sequence-scrambled siRNA (si-NC) were purchased from GenePharma Co. (Shanghai, China). Stable suppression of FEZF1-AS1 was performed by short-hairpin RNA (sh-RNA) interference. All oligonucleotide sequences are listed in Table S2 (see Additional file 2). The details of transfection and infection procedures are described in Supplementary Methods.

For transient knockdown experiments, the siRNAs, CXCR4 siRNA (si-CXCR4), and synthetic sequence-scrambled siRNA (si-NC), were purchased from GenePharma Co. (Shanghai, China). The stable suppression of SATB-1 was performed by short hairpin RNA interference. All oligonucleotide sequences are provided in Table S2 (see Additional file 2). The transfection and infection procedures are described in the Supplementary Methods.

For the transient knockdown assay, the small interfering RNAs (siRNAs), AURKA siRNA (siAURKA), NPM1 siRNA (siNPM1), YAP1 siRNA (siYAP1), and synthetic sequence-scrambled siRNA (siNC) were obtained from GenePharma (Shanghai, China). The short hairpin RNAs (shRNAs) of AURKA, NPM1, and YAP1 were purchased from GeneCopoeia (Guangzhou, China). The transfection and infection procedures were conducted following the manufacturer’s instructions. Stable cell lines were selected using the appropriate antibiotics for at least 48 h after transfection. The efficiency verification of siRNA, shRNA, and plasmid is in Fig. S2.