Colitis was induced in 12-weeks-old female C57BL/6 mice (Harlan laboratories, Israel) using dextran sodium sulfate (DSS). Mice were fed for 10 days with 1.5% (wt/vol) DSS in the drinking water. To assess the selectivity of mmRNA expression, IL10 or fLuc mmRNA loaded LNPs were self-assembled with ASSET and αLy6C or Isotype control antibodies and were injected intravenously on day 5 from DSS treatment (1 mg/kg). On day 6, mice were injected intraperitoneal with 200 μL of 15 mg/mL XenoLight d -luciferin substrate (PerkinElmer, USA) and anesthetized using isoflurane (Piramal, Israel). Luminescence levels were assessed and analyzed for whole mice and different organs using IVIS SpectrumCT in vivo imaging system and Living Image software (PerkinElmer, USA). Colon and spleen samples were homogenized to assess cytokines by IL-6 and IL10 ELISA kits (R&D Systems, USA).
Xenolight d luciferin substrate
Manufactured by PerkinElmer
Sourced in United States
XenoLight d-luciferin substrate is a bioluminescent substrate used in in vivo and in vitro research applications. It serves as a substrate for luciferase enzymes, enabling the detection and quantification of luciferase-expressing cells or organisms.
Automatically generated - may contain errors
3 protocols using xenolight d luciferin substrate
1
Selective Cytokine Modulation in Colitis Mice
2
In Vivo Bioluminescence Imaging
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Bioluminescence imaging of live animals was performed with an IVIS Lumina LT series III system (Perkin Elmer, Waltham, MA) as previously described (16 (link)). Infected mice were anesthetized with isoflurane, and Xenolight d -luciferin substrate (Perkin Elmer) was injected i.p. (150 μg/g body weight) 8 to 10 min prior to live imaging. Animals remained under isoflurane sedation for the duration of each imaging session, which occurred at various times postinfection until death or total virus clearance was achieved. Luminescent exposures were collected for 1 to 60 s with small or medium binning factors and various f-stop settings. Living Image Software (Perkin Elmer) was used for acquisition and analysis. For photon flux, a single region of interest was drawn around the entire body, and light emission was measured in photons per second per square centimeter per steradian.
3
In Vivo Bioluminescence Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Bioluminescence imaging of live animals was performed with an IVIS Lumina LT Series III system (Perkin Elmer, Waltham, MA). Infected mice were anesthetized with isoflurane and Xenolight D-luciferin substrate (Perkin Elmer, Waltham, MA) was injected intraperitoneally (150 μg/g body weight) 10 min prior to live imaging. Animals remained under isoflurane sedation for the duration of each imaging session which occurred at various timepoints post-infection until death or total viral clearance was achieved. Luminescent exposures were collected for 1–60 s with small or medium binning factors and various f-stop settings. Living Image Software (Perkin Elmer) was used for acquisition and analysis. For photon flux, a single region of interest was drawn around the entire body and light emission was measured in photons/sec/cd2/sr.
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