Horseradish peroxidase hrp conjugated anti rabbit igg antibodies

Virus titers were expressed as IFU/mL, which was determined using Madin-Darby canine kidney (MDCK) cells (kindly provided by Dr. Takaaki Nakaya, Kyoto Prefectural University of Medicine) as follows. MDCK monolayer cells were inoculated with a 10-fold serial dilution of each sample of interest for 14 hours at 37°C. The cells were then fixed with 4% paraformaldehyde (PFA), permeabilized with 0.3% Triton-X100 in PBS, and immunostained with anti-NP monoclonal antibodies (GeneTex). Signals were visualized using horseradish peroxidase (HRP)-conjugated anti-rabbit IgG antibodies (GE Healthcare) and TrueBlue Peroxidase Substrate (KPL, Gaithersburg, MD). IFU/mL was defined as the number of the cells positive for the anti-NP signals in 1 mL of each sample.

Virus titers were expressed as IFU/mL. IFU/mL was determined using Madin-Darby canine kidney (MDCK) cells as follows. MDCK monolayer cells were incubated with 10-fold serial dilutions of each sample of interest for 14 h at 37°C. The cells were then fixed with 4% paraformaldehyde, permeabilized with 0.3% Triton-X100 in PBS, and immunostained with anti-NP monoclonal antibodies (GeneTex, Irvine, CA). Signals were visualized using horseradish peroxidase (HRP)-conjugated anti-rabbit IgG antibodies (GE Healthcare, Waukesha, WI) and True Blue Peroxidase Substrate (KPL, Gaithersburg, MD). IFU/mL was defined as the number of the cells positive for the anti-NP signals in 1 mL of each sample.