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Polyacrylamide 0.5 criterion precast tbe gel

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The 6% polyacrylamide 0.5×Criterion precast TBE gel is a laboratory equipment product designed for electrophoresis applications. It is a precast gel that uses Tris-Borate-EDTA (TBE) buffer system. The gel has a concentration of 6% polyacrylamide.

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Lab products found in correlation

Lightshift chemiluminescent emsa kit, by Thermo Fisher Scientific (2 mentions) Ne per nuclear and cytoplasmic extraction kit, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Polyacrylamide gel (457 citations) Precast gels (207 citations) 4–20% precast polyacrylamide gel (178 citations) Criterion gels (84 citations) Criterion precast gels (71 citations) 5% TBE gel (14 citations) Criterion polyacrylamide gels (12 citations) TBE polyacrylamide gel (6 citations) TBE precast gel (3 citations)

2 protocols using polyacrylamide 0.5 criterion precast tbe gel

1

Elucidating Transcription Factor Binding Patterns

Check if the same lab product or an alternative is used in the 5 most similar protocols
EMSAs were performed using the LightShift Chemiluminescent EMSA Kit
(Thermo Scientific) and single-stranded biotinylated oligonucleotides and
complementary sequences corresponding to 31 nucleotides (15 nucleotides flanking
the SNP of interest). Nuclear extract from Jurkat, HH, and Daudi cells was
isolated using the NE-PER nuclear and cytoplasmic extraction kit (Thermofisher
Scientific) with slight modification. Binding reactions were incubated at room
temperature for 30 min and loaded onto a 6% polyacrylamide 0.5×
Criterion precast TBE gel (Biorad). After sample electrophoresis and transfer to
a nylon membrane, DNA was crosslinked for 10 min, and biotinylated probes
detected by chemiluminescence followed by film exposure. Details in Supplementary Note, Supplementary Figures,
Supplementary Tableand Source Data files.
Gutierrez-Arcelus M., Baglaenko Y., Arora J., Hannes S., Luo Y., Amariuta T., Teslovich N., Rao D.A., Ermann J., Jonsson A.H., Navarrete C., Rich S.S., Taylor K.D., Rotter J.I., Gregersen P.K., Esko T., Brenner M.B, & Raychaudhuri S. (2020). Allele-specific expression changes dynamically during T cell activation in HLA and other autoimmune loci. Nature genetics, 52(3), 247-253.
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2

Elucidating Transcription Factor Binding Patterns

Check if the same lab product or an alternative is used in the 5 most similar protocols
EMSAs were performed using the LightShift Chemiluminescent EMSA Kit
(Thermo Scientific) and single-stranded biotinylated oligonucleotides and
complementary sequences corresponding to 31 nucleotides (15 nucleotides flanking
the SNP of interest). Nuclear extract from Jurkat, HH, and Daudi cells was
isolated using the NE-PER nuclear and cytoplasmic extraction kit (Thermofisher
Scientific) with slight modification. Binding reactions were incubated at room
temperature for 30 min and loaded onto a 6% polyacrylamide 0.5×
Criterion precast TBE gel (Biorad). After sample electrophoresis and transfer to
a nylon membrane, DNA was crosslinked for 10 min, and biotinylated probes
detected by chemiluminescence followed by film exposure. Details in Supplementary Note, Supplementary Figures,
Supplementary Tableand Source Data files.
Gutierrez-Arcelus M., Baglaenko Y., Arora J., Hannes S., Luo Y., Amariuta T., Teslovich N., Rao D.A., Ermann J., Jonsson A.H., Navarrete C., Rich S.S., Taylor K.D., Rotter J.I., Gregersen P.K., Esko T., Brenner M.B, & Raychaudhuri S. (2020). Allele-specific expression changes dynamically during T cell activation in HLA and other autoimmune loci. Nature genetics, 52(3), 247-253.
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