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Dipotassium edta

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Dipotassium EDTA is a chemical compound used as an anticoagulant in blood collection and sample preparation. It acts by binding to calcium ions, preventing the coagulation of blood samples.

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Lab products found in correlation

Microtainer tube, by BD (1 mentions) Il 10, by BioLegend (1 mentions) Tgf β, by Thermo Fisher Scientific (1 mentions) Hdl and ldl vldl quantitation kit, by Merck Group (1 mentions) Elisa assays, by BioLegend (1 mentions) Prism software v6, by GraphPad (1 mentions) 7900ht fast real time pcr system, by Thermo Fisher Scientific (1 mentions) Nucleospin plasma kit, by Macherey-Nagel (1 mentions) Prism software, by GraphPad (1 mentions) Taqman qpcr assay, by Thermo Fisher Scientific (1 mentions) Nucleospin plasma xs kit, by Macherey-Nagel (1 mentions)

Spelling variants of this product

BD Microtainer tube with Dipotassium EDTA (2 citations) Dipotassium EDTA tubes (2 citations) Microtainer tubes with dipotassium EDTA (2 citations)

4 protocols using dipotassium edta

1

Cholesterol and Cytokine Analysis in Mice

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After 8 weeks treatment, mice were euthanized, and blood was collected by retro-orbital puncture with BD Microtainer tubes and dipotassium EDTA (BD Biosciences). Serum was separated by centrifugation at 3000 rpm at 4°C for 25min and stored at −80C. Total cholesterol was determined by HDL and LDL/VLDL Quantitation Kit (Sigma). Cytokines TGF-β (ThermoFischer Scientific) and IL-10 (Biolegend) were measured by ELISA assays (BioLegend) and cytokines (IL-6, IFN-γ) were measured by a customized Luminex Multiplex panel, according to the manufacturer’s instructions (ThermoFisher Scientific).
Yi S., Zhang X., Sangji H., Liu Y., Allen S.D., Xiao B., Bobbala S., Braverman C.L., Cai L., Hecker P.I., DeBerge M., Thorp E.B., Temel R.E., Stupp S.I, & Scott E.A. (2019). Surface engineered polymersomes for enhanced modulation of dendritic cells during cardiovascular immunotherapy. Advanced functional materials, 29(42), 1904399.
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2

Quantification of Circulating Cell-Free DNA

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Serial blood samples (250 μL) were collected by retroorbital bleeding from PDX mice using microtainer tubes with dipotassium EDTA (BD Biosciences, San Jose, CA). cfDNA was isolated from 100 µL of the plasma samples using the NucleoSpin Plasma kit (Macherey-Nagel, Düren, Germany). cfDNA concentration in the plasma was subsequently determined by quantitative polymerase chain reaction using the Custom TaqMan assay with the 7900HT Fast Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA). Human β-actin primer pair and probe set are as follows: forward primer, 5′-ATCCTAAAAGCCACCCCACT-3′; reverse primer, 5′-CTCAAGTTGGGGGACAAAAA-3′; and probe, 5′-FAM-CACAGGGGAGGTGATAGCAT-MGB-3′. Serial dilutions of genomic DNA extracted from the Hut78 CTCL cell lines were used to calibrate for cfDNA quantification. Sample cfDNA concentrations were extrapolated from the standard curve using Prism software v.6.0 (GraphPad Software, La Jolla, CA).
Wu C.H., Wang L., Yang C.Y., Wen K.W., Hinds B., Gill R., McCormick F., Moasser M., Pincus L, & Ai W.Z. (2022). Targeting CD70 in cutaneous T-cell lymphoma using an antibody-drug conjugate in patient-derived xenograft models. Blood Advances, 6(7), 2290-2302.
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3

Quantification of Plasma Cell-Free DNA

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Serial blood samples (250 μl) were collected by retro-orbital bleeding from PDX mice in microtainer tubes with dipotassium EDTA (BD Biosciences, San Jose, CA). cfDNA was purified from 100 μl of the plasma samples using the NucleoSpin Plasma XS kit (Macherey-Nagel, Bethlehem, PA) according to the manufacturer′s instructions. Plasma cfDNA concentrations were subsequently determined by TaqMan qPCR assay (Applied Biosystems, Waltham, MA). The following human β-actin primer pair and probe set were used: forward primer 5′-ATCCTAAAAGCCACCCCACT-3′; reverse primer 5′-CTCAAGTTGGGGGACAAAAA-3′; and probe 5′-FAM-CACAGGGGAGGTGATAGCAT-TAMURA-3′. Serial dilutions of genomic DNA extracted from the Hut78 and H9 CTCL cell lines were used to calibrate for cfDNA quantification. cfDNA concentrations were extrapolated from the standard curve using Prism software, version 6.0 (GraphPad Software, La Jolla, CA).
Hutchinson J., Jin J., Cardiff R.D., Woodgett J.R, & Muller W.J. (2001). Activation of Akt (Protein Kinase B) in Mammary Epithelium Provides a Critical Cell Survival Signal Required for Tumor Progression. Molecular and Cellular Biology, 21(6), 2203-2212.
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4

Ethanol Metabolism Kinetics in Mice

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To rule out the possibility of the α5 gene deletion directly altering ethanol metabolism, we assessed BEC in drug-naïve α5 KO and WT mice over a two-hour time course following administration of a single high dose of ethanol (4.0 g/kg, i.p.). Blood was collected into microtainers containing dipotassium EDTA (BD Biosciences, San Jose, CA, USA) via submandibular cheek punch at 15-, 30-, 60- or 120 min time-points after injection. Samples were prepared for analysis by aliquoting 20 µl of whole blood into 20 ml GC vials (Autosampler Guys, Alexandria, VA) containing 960 µl deionized (DI) water and 20 µl of 0.1 mg/ml 1-propanol standard. Vials were capped and stored at −20 ˚C until BECs were analyzed using headspace gas chromatography as described (Wolstenholme et al., 2011 ).
Dawson A., Wolstenholme J.T., Roni M.A., Campbell V.C., Jackson A., Slater C., Bagdas D., Perez E.E., Bettinger J.C., De Biasi M., Miles M.F, & Damaj M.I. (2018). Knockout of alpha 5 nicotinic acetylcholine receptors subunit alters ethanol-mediated behavioral effects and reward in mice.. Neuropharmacology, 138, 341-348.
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