The cell lines MCF7, HT29, SKBR3, H441, H1355, H1993, H1648, A549, A431, H820, PC9, HCC193, H1975, H2882 and H1650 were purchased from the American Type Culture Collection (Manassas, VA) or donated by other labs. Although the cell lines were not authenticated by our lab, the application herein does not require authentication since the lines are used as standards, not at biological models. Cell lines were selected to represent a range of EGFR expression and different mutation status. H1648, A549, H1355 were routinely cultured in Dulbecco’s modified Eagle medium: Nutrient Mixture F-12 containing 10% fetal bovine serum and 1% penicillin – streptomycin (Life Technologies, Inc., Grand Island, NY). A431 and MCF7 cells were cultured in Dulbecco’s modified Eagle medium containing 10% fetal bovine serum and 1% penicillin – streptomycin (Life Technologies, Inc., Grand Island, NY). H820, H1993, H441, H2882, HCC193, PC9, H1650, H1975 cells were cultured in RPMI 1640 medium containing 10% fetal bovine serum and 1% penicillin – streptomycin (Life Technologies, Inc., Grand Island, NY). HT29 and SKBR3 were cultured in McCoys medium containing 10% fetal bovine serum and 1% penicillin – streptomycin (Life Technologies, Inc., Grand Island, NY).
H1648
Manufactured by Thermo Fisher Scientific
The H1648 is a laboratory equipment product from Thermo Fisher Scientific. It is designed for specific laboratory applications, but a detailed unbiased description is not available at this time.
Automatically generated - may contain errors
Lab products found in correlation
H1993, by Thermo Fisher Scientific (2 mentions)
Mcf 7, by Thermo Fisher Scientific (2 mentions)
H1975, by Thermo Fisher Scientific (2 mentions)
H1650, by Thermo Fisher Scientific (2 mentions)
Sk br 3, by Thermo Fisher Scientific (2 mentions)
H1355, by Thermo Fisher Scientific (2 mentions)
H2882, by Thermo Fisher Scientific (2 mentions)
Hcc193, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Mcf 7, by American Type Culture Collection (2 mentions)
Skbr3, by American Type Culture Collection (2 mentions)
H1975, by American Type Culture Collection (2 mentions)
H1650, by American Type Culture Collection (2 mentions)
H1993, by American Type Culture Collection (2 mentions)
H1355, by American Type Culture Collection (2 mentions)
H1648, by American Type Culture Collection (2 mentions)
Hcc193, by American Type Culture Collection (2 mentions)
H2882, by American Type Culture Collection (2 mentions)
2 protocols using h1648
1
Cell Line Culturing Conditions
2
Cell Line Culturing Conditions
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The cell lines MCF7, HT29, SKBR3, H441, H1355, H1993, H1648, A549, A431, H820, PC9, HCC193, H1975, H2882 and H1650 were purchased from the American Type Culture Collection (Manassas, VA) or donated by other labs. Although the cell lines were not authenticated by our lab, the application herein does not require authentication since the lines are used as standards, not at biological models. Cell lines were selected to represent a range of EGFR expression and different mutation status. H1648, A549, H1355 were routinely cultured in Dulbecco’s modified Eagle medium: Nutrient Mixture F-12 containing 10% fetal bovine serum and 1% penicillin – streptomycin (Life Technologies, Inc., Grand Island, NY). A431 and MCF7 cells were cultured in Dulbecco’s modified Eagle medium containing 10% fetal bovine serum and 1% penicillin – streptomycin (Life Technologies, Inc., Grand Island, NY). H820, H1993, H441, H2882, HCC193, PC9, H1650, H1975 cells were cultured in RPMI 1640 medium containing 10% fetal bovine serum and 1% penicillin – streptomycin (Life Technologies, Inc., Grand Island, NY). HT29 and SKBR3 were cultured in McCoys medium containing 10% fetal bovine serum and 1% penicillin – streptomycin (Life Technologies, Inc., Grand Island, NY).
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