Anti mhcii apc cy7

Manufactured by Thermo Fisher Scientific

The Anti-MHCII-APC-Cy7 is a fluorescently-labeled antibody used in flow cytometry applications. It binds to the major histocompatibility complex class II (MHCII) proteins expressed on the surface of antigen-presenting cells. The APC-Cy7 fluorophore conjugated to the antibody enables detection and analysis of MHCII-positive cells using appropriate flow cytometry instrumentation and software.

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MHCII (84 citations) Anti-MHCII (34 citations) APC-Cy7 (32 citations) MHCII-APC (11 citations) Anti-MHCII-APC (9 citations) MHCII-APC-Cy7 (2 citations)

3 protocols using anti mhcii apc cy7

Multiparameter Flow Cytometry Myeloid and Lymphocyte Panel

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Cell suspensions were Fc-Receptor blocked and stained with the following antibodies (all from Biolegend unless indicated otherwise): (Myeloid panel) anti-Ly6G-FITC (1A8; 1.25 μg/ml), anti-CD64-PE (X54-5/7.1; 2 μg/ml), anti-CD11c-PerCP-Cy5.5 (N418; 1 μg/ml), anti-CD11b-PE-Cy7 (M1/70; 0.2 μg/ml), anti-Ly6C-AlexaFluor700 (HK1.4; 2 μg/ml), anti-MHCII-APC-Cy7 (M5/114.15.2; 0.067 μg/ml), anti-TREM-1-eFluor660 (TR3MBL1 from eBioscience; 0.2 μg/ml), anti-CD45-BrilliantViolett (30-F11; 1 μg/ml). (Lymphocyte panel) anti-CD8b-FITC (53-5.8; 0.8 μg/ml), anti-TCRab-PE, anti-GL7-PerCP-Cy5.5 (GL7; 1 μg/ml), anti-CD19-PE-Cy7 (6D5; 0.5 μg/ml), anti-CD11b-PacificBlue (M1/70; 0.25 μg/ml), anti-NK1.1-AlexaFluor700 (PK136; 2.5 μg/ml), anti-CD4-APC-Cy7 (RM4-5; 0.25 μg/ml), anti-BTLA-AlexaFluor647 (6A6; 2.5 μg/ml), anti-CD45-BrilliantViolett (30-F11; 1 μg/ml). As an isotype control for the anti-TREM-1-e-Fluor660 a mouse anti rat IgG2a-eFluor660 antibody (eBR2a; 0.2 μg/ml) from eBioscience was used. Dead cells were excluded using DAPI (Invitrogen) at a final concentration of 0.5 μg/ml. All samples were acquired on a LSRII SORP (BD Biosciences, San Diego, CA) and analyzed using the FlowJo Software (Tree Star, Ashland, OR).

Saurer L., Zysset D., Rihs S., Mager L., Gusberti M., Simillion C., Lugli A., Zlobec I., Krebs P, & Mueller C. (2017). TREM-1 promotes intestinal tumorigenesis. Scientific Reports, 7, 14870.

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Comprehensive Flow Cytometry Immunophenotyping

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Flow cytometry was performed as described previously^{23 (link)}, with the following antibodies: anti-IL-4-APC (11B11), anti-H-2K^b-APC (AF6-88.5.5.3), anti-CD70-PE (FR70), anti-TCRβ-APC-CY7 (H57-597), anti-IFN-γ-PE-CY7 (XMG1.2), anti-CD44-APC (IM7), anti-CD62L-PE-CY7 (MEL-14), anti-CD44-APC-CY7 (IM7), anti-CD44-FITC (IM7), anti-CD11c-PE (N418), anti-CD205-APC (205yekta), anti-CD40-PE (1C10), anti-MHC-II-APC-CY7 (M5/114.15.2), anti-CD80-APC (16-10A1), anti-CD86-FITC (GL1), anti-4-1BBL-PE (TKS-1), anti-OX40L-APC (RM134L), anti-PD-L1-PE (MIH5), anti-LAG3-PE (eBioC9B7W) and anti-CD11c-PE-CY7 (N418) (all from eBioscience); anti-CD44-BV650 (IM7), anti-CD11b-BV650 (M1/70), anti-IL-17A-BV421 (TC11), anti-PD-1-BV421 (29F.1A12), anti-TIM3-APC (B8.2c12) and anti-CD4-BV711 (RM4-5) (all from Biolegend); anti-IL-2-PE (JES6-5H4, BD Biosciences); and anti-CD8-BV605 (53-6.7, Sony Biotechnology Inc). For intracellular cytokine detection, cells were stimulated for 4 to 5 h with phorbol 12-myristate 13-acetate (PMA) and ionomycin in the presence of monensin before staining according to the manufacturer’s instructions (BD Biosciences). Mitotracker and Tetramethylrhodamine, methyl ester (TMRM) staining was performed according to the manufacturer’s instructions (Invitrogen). Flow cytometry data were acquired on LSRII or LSR Fortessa (BD Biosciences) and analyzed using FlowJo software (Tree Star).

Du X., Wen J., Wang Y., Karmaus P., Khatamian A., Tan H., Li Y., Guy C., Nguyen T.L., Dhungana Y., Neale G., Peng J., Yu J, & Chi H. (2018). Hippo/Mst signaling couples metabolic state and immune function of CD8α+ dendritic cells. Nature, 558(7708), 141-145.

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Collagen-Induced Arthritis Treatment Evaluation

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Ten-week-old male DBA1 mice (Charles River) were immunized to induce CIA as previously described [23 (link)]. Mice were treated intraperitoneally with CEP-701 (20 mg/kg, LC laboratories) or vehicle solution every 12 h for 15 consecutive days. LNs and paws were collected for further examination. Single-cell suspensions were obtained and stained using the following fluorochrome-conjugated antibodies: anti-CD11c (PE-Cy7, eBioscience), anti-MHCII (APC-Cy7, eBioscience), anti-CD11b (Alexa 700), anti-CD103 (FITC, eBioscience), anti-CD8 (eFluor780, eBioscience), anti-CD3 (Alexa 700, eBioscience) and anti-CD45 (PE, eBioscience).

Ramos M.I., Garcia S., Helder B., Aarrass S., Reedquist K.A., Jacobsen S.E., Tak P.P, & Lebre M.C. (2020). cDC1 are required for the initiation of collagen-induced arthritis. Journal of Translational Autoimmunity, 3, 100066.

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