Cell lysates were separated by 10% SDS-PAGE. The specific primary antibodies used in Western blotting analysis are as follows: FAP (1:1000, ab53066, Abcam, UK), α-SMA (1:1000, ab5694, Abcam), IL11 (1:1000, ab89887, Abcam), IL15 (1:800, ab7213, Abcam); VEGFA (1:1000, wl00009b, Wanleibio, China); p-VEGFR2 (1:800, D155165, Sangon, China), T-VEGFR2 (1:1000, D151118, Sangon), p-KIT (1:800, D151515, Sangon), p-PDGFRβ (1:800, D151409, Sangon); FXR1 (1:1000, BS70701, bioworld, China); YAP1 (1:1000, sc-398182, Santa Cruz, USA); T-STAT3 (1:1000, #4904, CST, USA), p-STAT3 (1:1000, #9131, CST), T-AKT (1:1000, #9272, CST), p-AKT (1:1000, #2965, CST), T-ERK (1:1000, #9102, CST), p-ERK (1:1000, #4348, CST), T-P38 (1:1000, #9212, CST), p-P38 (1:1000, #4631, CST) and β-Actin (1:1500, ZSBIO, China). For ELISA assay, conditioned medium was collected from 1 × 106 NFs or CAFs in a 6-well plate, and the concentrations of IL11 and IL15 were measured using a standard ELISA Kit (Raybiotech, USA) according to the manufacturer's instructions.
Ab89887
Manufactured by Abcam
Sourced in United Kingdom, China
Ab89887 is a laboratory equipment product. It is designed to perform a specific function in a laboratory setting. No further details on the core function of this product can be provided in an unbiased and factual manner.
Automatically generated - may contain errors
Lab products found in correlation
Ab53066, by Abcam (1 mentions)
Ab7213, by Abcam (1 mentions)
Sc 398182, by Santa Cruz Biotechnology (1 mentions)
Vegfa, by Wanlei (1 mentions)
Wl00009b, by Wanlei (1 mentions)
Ab5694, by Abcam (1 mentions)
β actin, by ZSGB-BIO (1 mentions)
Lncap, by American Type Culture Collection (1 mentions)
Du145, by American Type Culture Collection (1 mentions)
Hek293t, by American Type Culture Collection (1 mentions)
Rwpe 1, by American Type Culture Collection (1 mentions)
2 protocols using ab89887
1
Pathway analysis of fibroblast activation
2
Prostate Cancer Cell Line Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human prostate epithelial cell line RWPE-1, the embryonic kidney cell line HEK-293 T, and the prostate cancer cell lines PC-3, LNCAP,22RV1,IE8 and DU145 were all acquired from American Type Culture Collection (ATCC, Manassas, Virginia, USA). The culture conditions for all cell lines adhered to previously established protocols [19 (link), 20 (link)]. Rigorous quality control was maintained, with all cell lines testing negative for mycoplasma contamination. Additionally, to ensure the authenticity and purity of the cell lines, short tandem repeat (STR) authentication was performed, confirming no misidentification or contamination (verified by IGE Biotechnology, Guangzhou, Guangdong, China).For in vitro studies, PC3 or DU145 PCa cells, were stimulated with recombinant human IL-11 (Abcam,ab89887) or recombinant human IL-11RΑ (rhIL-11RΑ 10 ng/ml;Abcam,ab132575) for the indicated group, replacing culture medium and stimulus every 24 h.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!