Prior to DNA and RNA transfections, NIH3T3 or B16-F1 cells were grown in 24- or 6-well plates for 24h. For expression of fluorescently-tagged proteins, cells were transfected with 250-1500ng of DNA using LipofectamineLTX with Plus reagent (Invitrogen). For RNA interference experiments, cells were transfected with 50nM siControl RNAs (Ambion), siWHIMP Stealth siRNAs (MSS210515, MSS210516, MSS277782), siControl1 RNAs (Sigma), siBrk1 (00028245), siN-WASP (00117645), or siJMY (00043711) using RNAiMAX (Invitrogen). Cells were incubated in growth media for 24h after DNA transfections and 48h after siRNA transfections. Cells grown in 6-well plates were collected and processed for immunoblotting, and cells grown on 12mm glass coverslips in 24-well plates were fixed using paraformaldehyde.
Sicontrol1 rnas
Manufactured by Merck Group
SiControl1 RNAs are a set of small interfering RNAs (siRNAs) designed for use as negative controls in gene silencing experiments. They do not target any known gene and are intended to serve as a baseline for comparison with experimental siRNA treatments.
Automatically generated - may contain errors
Lab products found in correlation
Rnaimax, by Thermo Fisher Scientific (2 mentions)
Sicontrol rnas, by Thermo Fisher Scientific (2 mentions)
Siwhimp stealth sirnas, by Merck Group (2 mentions)
Lipofectamine ltx, by Thermo Fisher Scientific (1 mentions)
Plus reagent, by Thermo Fisher Scientific (1 mentions)
Lipofectamine ltx with plus reagent, by Thermo Fisher Scientific (1 mentions)
2 protocols using sicontrol1 rnas
1
NIH3T3 and B16-F1 Transfection Protocols
2
Fluorescent Protein Expression and RNAi in NIH3T3 and B16-F1 Cells
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Prior to DNA and RNA transfections, NIH3T3 or B16-F1 cells were grown in 24 or 6-well plates for 24h. For expression of fluorescently-tagged proteins, cells were transfected with 250-1500ng of DNA using LipofectamineLTX with Plus reagent (Invitrogen). For RNA interference experiments, cells were transfected with 50nM siControl RNAs (Ambion), siWHIMP Stealth siRNAs (MSS210515, MSS210516, MSS277782), siControl1 RNAs (Sigma), siBrk1 (00028245), siN-WASP (00117645), or siJMY (00043711) using RNAiMAX (Invitrogen). Cells were incubated in growth media for 24h after DNA transfections and 48h after siRNA transfections. Cells grown in 6-well plates were collected and processed for immunoblotting, and cells grown on 12mm glass coverslips in 24-well plates were fixed using paraformaldehyde.
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