Provisc

Example 18

To evaluate the aggregability of STMP following intravitreal injection ex vivo, enucleated cow eyes (J.W. Treuth & Sons, Catonsville, Md.) were utilized. The eyes were kept on ice prior to use. Briefly, 30 μL of 200 mg/mL STMP, S-10, suspended in 5-fold diluted ProVisc® was injected into the central vitreous of cow eyes using a 0.5 mL insulin syringe (Terumo) with a 27-gauge needle and three injections were performed in each cow eye at different locations. After a 2 hour incubation at 37° C., the eyes were cut open and the aggregates of STMP were examined using a dissecting microscope. As shown in FIG. 9, the injected STMP formed consolidated aggregates in cow vitreous and no apparent particle dispersion was observed.

Example 18

To evaluate the aggregability of STMP following intravitreal injection ex vivo, enucleated cow eyes (J.W. Treuth & Sons, Catonsville, Md.) were utilized. The eyes were kept on ice prior to use. Briefly, 30 μL of 200 mg/mL STMP, S-10, suspended in 5-fold diluted ProVisc® was injected into the central vitreous of cow eyes using a 0.5 mL insulin syringe (Terumo) with a 27-gauge needle and three injections were performed in each cow eye at different locations. After a 2 hour incubation at 37° C., the eyes were cut open and the aggregates of STMP were examined using a dissecting microscope. As shown in FIG. 9, the injected STMP formed consolidated aggregates in cow vitreous and no apparent particle dispersion was observed.

Example 18

To evaluate the aggregability of STMP following intravitreal injection ex vivo, enucleated cow eyes (J. W. Treuth & Sons, Catonsville, Md.) were utilized. The eyes were kept on ice prior to use. Briefly, 30 μL of 200 mg/mL STMP, S-10, suspended in 5-fold diluted ProVisc® was injected into the central vitreous of cow eyes using a 0.5 mL insulin syringe (Terumo) with a 27-gauge needle and three injections were performed in each cow eye at different locations. After a 2 hour incubation at 37° C., the eyes were cut open and the aggregates of STMP were examined using a dissecting microscope. As shown in FIG. 9, the injected STMP formed consolidated aggregates in cow vitreous and no apparent particle dispersion was observed.