24 well transwell permeable supports with 8 mm pores

A cell migration assay was performed by utilizing 24-well transwell permeable supports with 8 mm pores (Corning, NY, USA). Cells were suspended in a serum-free medium and seeded into the transwell embeds. The bottom wells were loaded with media containing complete media. After 24 h, cells were stained with 4 mg/mL calcein AM (Invitrogen, Carlsbad, CA, USA) in PBS at 37 °C for 1 h and detached from inserts by trypsinization. The fluorescence of the migrated cells was read using an Ultra Multifunctional Microplate Reader (TECAN, Durham, NC, USA). The cells were grown in the presence and absence of curcumin (25 µM) with or without neocuproine (50 µM).

The migration of cells was measured using 24-well transwell permeable supports with 8-mm pores (Corning), as previously described [40 (link)]. Using ULTRA Multifunctional Micro-plate Peruser (TECAN), we analyzed the migrated cells by reading their fluorescence. Cells were cultured at the indicated concentrations with and without neocuprione and isoflavones.

A cell migration assay was conducted using 24-well transwell permeable supports with 8 mm pores (Corning, NY, USA). The transwell embeds were planted with cells suspended in a solution devoid of serum. The lower wells were filled with media. After 24 hours, cells were stained with 4 mg/mL calcein AM (Invitrogen, Carlsbad, CA, USA) in PBS at 37 C for 1 hour and then detached from the inserts using trypsin. The fluorescence of the migrating cells was measured utilizing the ULTRA Multifunctional Microplate Peruser (TECAN, Durham, NC, USA). Cells were cultured in the presence and absence of delphinidin (50 µM) and neocuprione (50 µM) at various concentrations.