Waters synapt g2hdms system

Manufactured by Waters Corporation

Sourced in United States

The Waters SYNAPT G2HDMS system is a high-resolution mass spectrometry platform designed for advanced analytical applications. It features a hybrid quadrupole time-of-flight (QTOF) mass analyzer, providing high-resolution accurate mass measurements for the identification and characterization of complex molecules.

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Lab products found in correlation

Waters acquity uplc hss t3 column, by Waters Corporation (2 mentions) Waters acquity uplc 1 class system, by Waters Corporation (1 mentions) Masslynx 4, by Waters Corporation (1 mentions) Waters acquity uplc system, by Waters Corporation (1 mentions)

2 protocols using waters synapt g2hdms system

Quantitative Metabolomics Analysis by UPLC-MS

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Chromatography was performed on a Waters Acquity UPLC I-Class system (Waters Corp., Milford, USA) with a Waters Acquity UPLC HSS T3 column (2.1 ×100 mm, i.d. 1.8 µm; Waters, USA) maintained at 40 ℃. The mobile phases consist of 0.1% (v/v) formic acid in water (A) and 0.1% (v/v) formic acid in acetonitrile (B). A gradient elution program was used as follows: 0–4 min, 2–6% B; 4–18 min, 6–20% B; 18–21 min, 20–24% B; 21–30 min, 24–35% B; 30–33 min, 35–98% B. The flow rate was set at 0.5 mL/min, and a 1-μL aliquot was set as the injection volume.
Mass spectrometry analysis was performed on a Waters SYNAPT G2HDMS system (Waters Corp., Milford, USA) equipped with an electrospray ionization (ESI) source in both positive and negative ion modes. The optimal parameters were set as follows: capillary voltage, 2 kV; cone voltage,40 V; resolvation gas (N₂) flow, 900 L/h; source temperature, 100 ℃; resolvation temperature, 450 ℃; scanning time and interval, 0.2 s; scan range, m/z 50–1500; trap collision energy, 20–50 eV; lock mass, [M+H]⁺ 556.2775 and [M−H]⁻554.2615. The data were collected in the MS^E continuum mode using Masslynx 4.1 software (Waters Corp., Milford, USA).

Jiang H., Chen J., Li X., Zhong Y.T., Kang L.P., Wang G., Yu M., Fu L.F., Wang P, & Xu H.Y. (2022). Systematic identification of chemical components in Fufang Shuanghua oral liquid and screening of potential active components against SARS-CoV-2 protease. Journal of Pharmaceutical and Biomedical Analysis, 223, 115118.

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UPLC-MS Analysis of Metabolites

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The ARD and standard solutions were centrifuged at 10,033 g for 10 min, and 2 μL aliquots were respectively injected into the Waters ACQUITY UPLC HSS T3 column (1.7 μm × 2.1 mm × 100 mm; Waters, MA, USA) at 15°C in a Waters ACQUITY UPLC system. The samples were eluted with the flow rate at 0.2 mL/min using acetonitrile (solvent A) and 0.1% v/v formic acid in water (solvent B). The linear gradient was as follows: 0–10 min, 0%–1% A; 10–15 min, 1%–3% A; 15–20 min, 3%–10% A; 20–25 min, 10%–15% A; 25–30 min, 15%–20% A; 30–35 min, 20%–30% A; 35–40 min, 30%–60% A; 40–45 min, 60%–80% A; 45–50 min, 80%–90% A; 50–55 min, 90%–100% A; and 55–60 min, 100%–0% A.
The mass spectra of the above eluents were acquired on the Waters SYNAPT G2 HDMS system (Waters Corp., USA) in positive ion mode by scanning over the m/z range of 50–1200. The following conditions were used: desolvation gas flow at 800 L/h and 450°C, cone gas flow at 50 L/h and 100°C, capillary voltage 3 kV, cone voltage 40 V, and scan time 0.5 s. Leucine enkephalin with an [M+H]⁺ ion at m/z 556.2771 was used as the lock mass. The Mass Lynx V 4.1 software was used for data analysis.

Chang X.Y., Wu J.S., Zhang F.Q., Li Z.Z., Jin W.Y., Wang J.X., Wang W.H, & Shi Y. (2022). A Strategy for Screening the Lipid-Lowering Components in Alismatis Rhizoma Decoction Based on Spectrum-Effect Analysis. Journal of Analytical Methods in Chemistry, 2022, 2363242.

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