3 deoxyglucosone 3 dg

Histone protein H3, 3-deoxyglucosone (3-DG), arginine, lysine, sodium azide, 2,4-dinitrophenyl hydrazine (DNPH), 9,10-phenanthrenequinone, dialysis tubing and other reagents/chemicals were acquired from Sigma Chemical Company, USA. NBT was purchased from Sisco Research Laboratories, India.

Four AGE standards, CML (>98%), CEL (>98%) and its isotopically labeled compounds d₄-CML (>98%) and d₄-CEL (>98%), were purchased from Toronto Research Chemicals Inc. (Toronto, ON, Canada). Glyoxal (GO) solution (approx. 40%), methylglyoxal (MGO) solution (approx. 40%), 3-Deoxyglucosone (3-DG) (95%) and o-phenylenediamine (OPD) (99.5%) were obtained from Sigma-Aldrich (Steinheim, Germany). NDMA and NDEA were obtained from J&K Scientific Ltd. (Beijing, China). Oasis MCX solid-phase extraction cartridges (6 mL, 150 mg) were purchased from Waters Co. (Milford, MA, USA). Sodium borohydride, sodium hydroxide, sodium borate, boric acid, disodium hydrogen phosphate, ascorbic acid and sodium dihydrogen phosphate were purchased from Shanghai Aladdin biochemical technology Co., Ltd. (Shanghai, China). Acetonitrile and methanol were purchased Merck Company (Darmstadt, Germany). Hydrochloric acid (37%), petroleum ether and hexane were purchased Xilong Scientific Co., Ltd. (Guangdong, China). Bond Elut QuEChERS extraction package and QuEChERS matrix dispersion purification package components were obtained from Agilent Technologies Co., Ltd. (Santa Clara, CA, USA). Raw pork was purchased from Rainbow Supermarket (Nanchang, China). Collagen enteric coating was purchased from North Products Food Co (Zhaozhuang, China).