Goat anti chicken alexa fluor 647

Manufactured by Jackson ImmunoResearch

Sourced in Panama

Goat anti-chicken Alexa Fluor 647 is a secondary antibody conjugated with the Alexa Fluor 647 fluorescent dye. It is designed to detect and bind to primary antibodies raised in chickens, allowing for fluorescent labeling and visualization of target proteins or cells.

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Lab products found in correlation

Goat anti rat alexa fluor 555, by Abcam (3 mentions) Goat anti rat alexa fluor 647, by Thermo Fisher Scientific (3 mentions) Lsm 710 confocal microscope, by Zeiss (3 mentions) Alexa fluor 488 goat anti rabbit, by Thermo Fisher Scientific (2 mentions) Lsm 710 axioobserver, by Zeiss (2 mentions) Rat anti mouse cd31 mec 13, by BD (2 mentions) Rat anti mouse cd117 2b8, by Thermo Fisher Scientific (2 mentions) Chicken polyclonal anti gfp, by Thermo Fisher Scientific (2 mentions) Donkey anti rat alexa fluor 555, by Abcam (2 mentions) Volocity software, by PerkinElmer (2 mentions) Stereo discovery v8 microscope, by Zeiss (1 mentions) Chicken anti gfp, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 goat anti rabbit, by Jackson ImmunoResearch (1 mentions) Mec13, by BD (1 mentions)

3 protocols using goat anti chicken alexa fluor 647

Whole-Mount Confocal Microscopy of Mice and Live-Imaging of Zebrafish

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For mice, whole-mount confocal microscopy was performed as described (Yokomizo et al. 2012 (link)). Embryos were stained with rat anti-CD31 (MEC13.3, BD Biosciences), rabbit anti-Runx (EPR3099, Abcam), chicken anti-GFP (Invitrogen Molecular Probes), and rat anti-F4/80 (CI: A3-1, Abcam) primary antibodies and goat anti-rat Alexa Fluor 555 (Abcam), goat anti-rabbit Alexa Fluor 488, goat anti-chicken Alexa Fluor 647 (Jackson Immunoresearch), and goat anti-rat Alexa Fluor 647 (Invitrogen Molecular Probes) secondary antibodies. Specimens were analyzed using a Zeiss LSM 710 confocal microscope with 25× objectives using multitrack sequential mode. The pinhole was set at 1 airy unit; for three-dimensional (3D) reconstructions, steps were 5 µm per z-section.
For zebrafish, fluorescent reporter embryos were treated as indicated in the text and live-imaged under tricaine anesthesia as previously described (North et al. 2007 (link)) using a Zeiss SteREO Discovery V8 microscope.

Li Y., Esain V., Teng L., Xu J., Kwan W., Frost I.M., Yzaguirre A.D., Cai X., Cortes M., Maijenburg M.W., Tober J., Dzierzak E., Orkin S.H., Tan K., North T.E, & Speck N.A. (2014). Inflammatory signaling regulates embryonic hematopoietic stem and progenitor cell production. Genes & Development, 28(23), 2597-2612.

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Microscopic Imaging of Embryonic Vasculature

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Embryos were prepared as previously described (Yokomizo et al., 2012 (link)). A Zeiss LSM 710 AxioObserver inverted confocal microscope with ZEN 2011 software was used to acquire Z-projections and single optical projections. Images were processed using Fiji software (Schindelin et al., 2012 (link)). 3-dimensional reconstructions were produced using Volocity software (PerkinElmer). The following primary antibodies were used; rat anti-mouse CD31 (Mec 13.3, BD Pharmingen, San Diego, CA), rat anti-mouse CD117 (2B8, eBioscience, San Diego, CA), chicken anti-GFP (polyclonal, Thermo Fisher Scientific, Waltham, MA), rabbit anti-mouse Prox1 (AngioBio, San Diego, CA) and rabbit anti-human/mouse Runx (EPR3099, Abcam, Cambridge, MA). Secondary antibodies used were goat anti-rat Alexa Fluor 647 (Invitrogen, Carlsbad, CA), goat-anti rat Alexa Fluor 555 (Abcam), donkey anti-rat Alexa Fluor 555 (Abcam), goat anti-chicken Alexa Fluor 647 (Jackson ImmunoResearch, West Grove, PA) and goat anti-rabbit Alexa Fluor 488 (Invitrogen).

Yzaguirre A.D, & Speck N.A. (2016). Extravascular endothelial and hematopoietic islands form through multiple pathways in midgestation mouse embryos. Developmental biology, 415(1), 111-121.

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Zebrafish Embryo Imaging and Analysis

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Embryos were prepared as previously described (Yokomizo et al., 2012 (link)). A Zeiss LSM 710 AxioObserver inverted confocal microscope with ZEN 2011 software was used to acquire Z-projections and single optical projections. Images were processed using Fiji software (Schindelin et al., 2012 (link)). 3-dimensional reconstructions were produced using Volocity software (PerkinElmer). The following primary antibodies were used; rat anti-mouse CD31 (Mec 13.3 , BD Pharmingen, San Diego, CA), rat anti-mouse CD117 (2B8, eBioscience, San Diego, CA), chicken anti-GFP (polyclonal, Thermo Fisher Scientific, Waltham, MA) and rabbit anti-human/mouse Runx (EPR3099, Abcam, Cambridge, MA). Secondary antibodies used were goat anti-rat Alexa Fluor 647 (Invitrogen, Carlsbad, CA), goat-anti rat Alexa Fluor 555 (Abcam), donkey anti-rat Alexa Fluor 555 (Abcam), goat anti-chicken Alexa Fluor 647 (Jackson ImmunoResearch, West Grove, PA) and goat anti-rabbit Alexa Fluor 488 (Invitrogen).

Yzaguirre A.D, & Speck N.A. (2016). Insights into blood cell formation from hemogenic endothelium in lesser-known anatomic sites. Developmental dynamics : an official publication of the American Association of Anatomists, 245(10), 1011-1028.

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