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2 protocols using α egfr

1

Comprehensive Antibody Evaluation for Cell Signaling

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Antibodies against α-Rab5a (#2143), α-Rab7a (#9367), α-Rab10 (#8127), α-EGFR (#4267), α-Phospho-EGF Receptor (Tyr1068) (#2234 S), α-Phospho-p38 MAPK (Thr180/Tyr182) (#2775 S), α-p38 MAPK (D13E1) XP Rabbit mAb (#8690), α-p44/42 MAPK (Erk1/2) (#695), α-IRG1 (#17805) were purchased from Cell Signaling Technology. Antibodies against α-HIF1-α (sc-53546), α-EGFR (sc-120), α-PKM2 (sc-365684), α-LDHA (sc-133123), α-MAPK14 (sc-11415), α-EGFR (sc-120), α-EEA1 (sc-137130), α-TGN38 (sc-166594), α-LAMP1 (sc-20011), α-PPARγ (sc-7273), α-SDHA (sc-390381), α-COX2 (sc-514489), α-COX4 (sc-517553), α-ATP5A (sc-136178) were purchased from Santa Cruz Biotechnology. Antibodies against α-HA (51064-2-AP, proteintech Group), α-FLAG (20543-1-AP, proteintech Group), APC anti-mouse CD11b (APC-65055, proteintech Group) were purchased from proteintech Group. Antibodies against APC anti-mouse iNOS (#85-17-5920-82, eBioscience), PE anti-mouse CD206 (MMR) Antibody (#85-12-2061-82, eBioscience), α-F4/80 (11-4801-82, FITC, eBioscience), PE anti-mouse Ly6G (#12-9668-82, eBioscience), α-CD14 (25-0149-42, FITC, eBioscience) were purchased from eBioscience. Antibodies against α-GAPDH (T0004), α-β-Tubulin (T0023), α-Phospho-PPAR gamma (Ser112) (AF3284), α-Phospho-PKM2 (AF7231) were purchased from Affinity Biosciences.
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2

Epidermal Growth Factor Receptor Signaling

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Dulbecco’s modified Eagle’s media (DMEM), Fetal bovine serum (FBS), Penicillin-Streptomycin, Trypsin-EDTA, Epidermal growth factor (EGF) (Peprotech), Piperonylic acid, Tyrphostin AG1478, Cyanogen bromide-activated-Sepharose® 4B, Thiazolyl Blue Tetrazolium Bromide (MTT), Hoechst 33342 (Sigma-Aldrich), G418 (Calbiochem), Cell counting kit-8 (CCK-8) (Dojindo), Dako fluorescent mounting medium (Dako), CellROX® Green Reagent (Life technologies), α-p-ERK T202/Y204, α-p-AKT S473, α-p-PLCγ1 Y783, α-ERK, α-AKT, α-PLCγ1, α-Egr-1 (CST), α-p-Tyr, α-EGFR, α-c-Fos, α-p-STAT3 S727, α-STAT3 (SantaCruz), α-FGFR2 (Abcam), α-GAPDH (Millipore), α-Actin (MP biomedicals), α-mouse IgG HRP conjugate (Thermo), and α-rabbit IgG HRP conjugate (Promega).
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