Western blot analyses were carried out as described previously [12 (link)]. Target proteins were detected with the primary antibodies listed above and horseradish peroxidase (HRP)-conjugated anti-rabbit IgG or anti-mouse IgG antibodies (GE Healthcare). The bands of target proteins were visualized using the ECL plus chemiluminescence system (GE Healthcare). Quantification of the visualized bands was carried out by ImageJ software, and the ratios to quantities in unirradiated samples were calculated.
Hrp conjugated anti rabbit igg or anti mouse igg antibodies
Manufactured by GE Healthcare
HRP-conjugated anti-rabbit IgG or anti-mouse IgG antibodies are secondary antibodies used in immunoassay techniques. They are labeled with horseradish peroxidase (HRP) enzyme, which can be detected through a colorimetric or chemiluminescent reaction. These antibodies recognize and bind to the primary antibodies raised in rabbit or mouse, respectively, and serve as detection reagents in various immunological applications.
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Lab products found in correlation
2 protocols using hrp conjugated anti rabbit igg or anti mouse igg antibodies
1
Western Blot Quantification Protocol
2
Western Blot Analysis Protocol
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Western blot analyses were carried out as described previously [26] . Target proteins were detected with the primary antibodies mentioned above and horseradish peroxidase (HRP)-conjugated anti-rabbit IgG or anti-mouse IgG antibodies (GE Healthcare). Proteins were visualized using the ECL plus chemiluminescence system (GE Healthcare).
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