Log In Sign up
The largest database of trusted experimental protocols

Anti human igg fitc

Manufactured by Merck Group
Visit Supplier
Sourced in United States

Anti-human IgG-FITC is a conjugated antibody used in various laboratory techniques, such as immunoassays and flow cytometry. It is composed of an anti-human IgG antibody labeled with the fluorescent dye FITC (Fluorescein Isothiocyanate). This product can be used to detect and quantify human immunoglobulin G (IgG) in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Anti mouse igg fitc, by Merck Group (3 mentions) Sheep anti human c3c fitc, by Bio-Rad (2 mentions) Lsm 880, by Zeiss (2 mentions) Cellmask orange, by Thermo Fisher Scientific (1 mentions) Anti baff r antibody clone 11c1, by BD (1 mentions) Anti human cd19, by BD (1 mentions) Nilotinib, by Novartis (1 mentions) Tcs sp5 2 confocal microscope, by Leica (1 mentions) Anti mouse igg fitc, by Santa Cruz Biotechnology (1 mentions) Mouse anti flag antibody, by Merck Group (1 mentions) Attune nxt, by Thermo Fisher Scientific (1 mentions) Facslyric, by BD (1 mentions) Sars cov 2 spike antibody, by Sino Biological (1 mentions) Alkaline phosphatase conjugated anti human igg, by Southern Biotech (1 mentions) Human serum albumin (hsa), by Merck Group (1 mentions) Vector blue substrate, by Vector Laboratories (1 mentions) Aec substrate kit, by Merck Group (1 mentions) Streptavidin hrp, by Bio-Rad (1 mentions) Spectramax m2, by Molecular Devices (1 mentions) Facscanto 2, by BD (1 mentions)

Close spelling variants of this product

FITC-labeled anti-human IgG antibody Anti-human IgG (Fc)-FITC Anti-human IgG (Fc specific)-FITC ( FITC)-conjugated monoclonal anti-human IgG detection antibody Anti-human IgG FITC-conjugated antibody Anti-human IgG-FITC (H + L) (Fab) Goat Anti-human IgG Fc-FITC antibody Goat anti-human IgG (Fab specific)-FITC antibody Goat FITC-conjugated anti-human IgG antibody Goat anti-Human IgG (Fc specific)-FITC antibody

12 protocols using anti human igg fitc

1

Blocking BAFF-R Signaling in B Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Nilotinib and anti-BAFF-R B-1239 antibodies (unconjugated and Alexa-647 labeled) were from Novartis. These antibodies were selected from the Human Combinatorial Antibody Library (HuCAL GOLD{r}), using phage display technology (8 (link)). The B-1239 mAb was produced in a fucosyl-transferase deficient CHO cell line (BioWa Potelligent Technology, BioWa Inc., Princeton, NJ, USA) (20 (link)), with a humanized sequence to optimalize ADCC activity. B-1239 was selected based on its property of blocking the BAFF/BAFF-R interaction and signaling via BAFF. Anti-BAFF-R antibody clone 11c1, anti-human CD19 and CD10 antibodies were from BD Biosciences (San Jose, CA, USA). FITC-anti-human IgG was from Sigma Aldrich (CA, USA). Recombinant huBAFF and the function-blocking anti-BAFF-R antibodies were purchased from R&D Systems (MN, USA). Bcr N-20, BAFF-R and Gapdh antibodies for Western blotting were from Santa Cruz, eBiosciences and Millipore, respectively. Cell Mask Orange and Deep Red were from Life Technologies (Eugene, OR).
Parameswaran R., Lim M., Fei F., Abdel-Azim H., Arutyunyan A., Schiffer I., McLaughlin M.E., Gram H., Huet H., Groffen J, & Heisterkamp N. (2014). Effector-mediated eradication of precursor B acute lymphoblastic leukemia with a novel Fc engineered monoclonal antibody targeting the BAFF-R. Molecular cancer therapeutics, 13(6), 1567-1577.
+ Open protocol
+ Expand
2

Immunofluorescence Analysis of Ig Deposition

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunofluorescence analysis was conducted to measure immunoglobulin deposition in human and mouse kidneys (29 (link)). FITC anti-human IgG (Sigma-Aldrich, St. Louis, MO, USA) and human IgE (eBioscience, San Diego, CA, USA), as well as FITC anti-mouse IgG (Santa Cruz Biotechnology, CA, USA) and IgE (eBioscience, San Diego, CA, USA) were employed. For human biopsy, the fluorescence intensity of glomerular staining was graded on a scale from 0 to ++++ in increments of 0.5+. Images were recorded under a TCS SP5 II confocal microscope (Leica Microsystems, Mannheim, Germany).
Pan Q., Gong L., Xiao H., Feng Y., Li L., Deng Z., Ye L., Zheng J., Dickerson C.A., Ye L., An N., Yang C, & Liu H.F. (2017). Basophil Activation-Dependent Autoantibody and Interleukin-17 Production Exacerbate Systemic Lupus Erythematosus. Frontiers in Immunology, 8, 348.
+ Open protocol
+ Expand
3

Quantifying SARS-CoV-2 ACE2 Binding

Check if the same lab product or an alternative is used in the 5 most similar protocols
Virus producing HEK293T cells were harvested 72 hrs after transfection. Cells were dissociated by incubation at 37°C in PBS + 5mM EDTA for 30 min. Then, cells were fixed in 4% formaldehyde in PBS, and stained with sACE2-humanFc fusion protein (construct is a gift of Jason McLellan, University of Texas at Austin) or mouse anti-Flag antibody (Sigma, F3165). Cells were then stained with anti-human-IgG-FITC (Sigma, F9512) or anti-mouse-IgG-FITC (Sigma, F0257) and processed by a Life Technologies Attune NxT flow cytometer. Results were processed using FlowJo v7.6.5 (Ashland, OR). Relative ACE2 binding was then determined by dividing sACE2 signal by anti-Flag signal measured by mean fluorescence intensity.
Zeng C., Evans J.P., Qu P., Faraone J., Zheng Y.M., Carlin C., Bednash J.S., Zhou T., Lozanski G., Mallampalli R., Saif L.J., Oltz E.M., Mohler P., Xu K., Gumina R.J, & Liu S.L. (2021). Neutralization and Stability of SARS-CoV-2 Omicron Variant. bioRxiv.
+ Open protocol
+ Expand
4

Flow Cytometry and Western Blot Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-human IgG–FITC and anti-mouse IgG-FITC were from Sigma-Aldrich and were used at a dilution of 1:100 in HBSS containing 0.15 mM CaCl2 and 1 mM MgCl2 (HBSS++) and 1% BSA (HBSS++/BSA) in flow cytometry assays. Goat anti-human FH, alkaline phosphatase conjugated anti-human IgG (Southern Biotechnology) and donkey anti-goat IgG were used in Western blots a dilution of 1:1000 in PBS with 5% non-fat dry milk. Anti-C4BP mAb 104 (34 (link), 35 (link)) was used in flow cytometry assays at a concentration of 10 μg/mL.
Shaughnessy J., Chabeda A., Tran Y., Zheng B., Nowak N., Steffens C., DeOliveira R.B., Gulati S., Lewis L.A., Maclean J., Moss J.A., Wycoff K.L, & Ram S. (2022). An optimized Factor H-Fc fusion protein against multidrug-resistant Neisseria gonorrhoeae. Frontiers in Immunology, 13, 975676.
+ Open protocol
+ Expand
5

Sheep anti-human C3c and IgG detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sheep anti-human C3c-FITC was obtained from AbD Serotec (cat. # AHP031F), and anti-human IgG FITC was from Sigma. Both antibodies (Abs) were used at a dilution of 1:200 in HBSS++ and 1% bovine serum albumin (BSA) (HBSS++/BSA) in flow cytometry assays.
Shaughnessy J., Lewis L.A., Zheng B., Carr C., Bass I., Gulati S., DeOliveira R.B., Gose S., Reed G.W., Botto M., Rice P.A, & Ram S. (2018). Human factor H domains 6 and 7 fused to IgG1 Fc are immunotherapeutic against Neisseria gonorrhoeae. Journal of immunology (Baltimore, Md. : 1950), 201(9), 2700-2709.
+ Open protocol
+ Expand
6

Flow Cytometry Antibody Labeling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-human IgG FITC (Sigma), anti-human C3c-FITC (BioRad), and anti-mouse C3 FITC (MP Biomedicals) were used in flow cytometry assays, all at a dilution of 1:100 in Hanks Balanced Salt Solution (HBSS) containing 0.1% BSA and 1 mM CaCl2 and 1 mM MgCl2 (HBSS++/BSA).
Wong S.M., Shaughnessy J., Ram S, & Akerley B.J. (2016). Defining the Binding Region in Factor H to Develop a Therapeutic Factor H-Fc Fusion Protein against Non-Typeable Haemophilus influenzae. Frontiers in Cellular and Infection Microbiology, 6, 40.
+ Open protocol
+ Expand
7

SARS-CoV-2 Spike Protein Cell Surface Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
In order to analyze transfected cells for cell surface expression of S protein, 2 × 105 cells of each of transfected and untransfected cells stained with SARS-CoV-2 Spike antibody (SinoBiological) and Anti-human IgG FITC (Sigma–Aldrich®). After staining with primary and secondary antibodies, cells were acquired on BD FACS Lyrics and analyzed by Flowjo V10 (BD Biosciences).
Alimohammadi R., Porgoo M., Eftekhary M., Kiaie S.H., Ansari Dezfouli E., Dehghani M., Nasrollahi K., Malekshahabi T., Heidari M., Pouya S., Alimohammadi M., Sattari Khavas D., Modaresi M.S., Ghasemi M.H., Ramyar H., Mohammadipour F., Hamzelouei F., Mofayezi A., Mottaghi S.S., Rahmati A., Razzaznian M., Tirandazi V., Tat M., Borzouee F., Sadeghi H., Haji Mohammadi M., Rastegar L., Safar Sajadi S.M., Ehsanbakhsh H., Bazmbar H., Baghernejadan Z., Shams Nouraei M., Pazooki P., Pahlavanneshan M., Alishah K., Nasiri F., Mokhberian N., Mohammadi S.S., Akar S., Niknam H., Azizi M., Ajoudanian M., Moteallehi-Ardakani M.H., Mousavi Shaegh S.A., Ramezani R., Salimi V., Moazzami R., Hashemi S.M., Dehghanizadeh S, & Khoddami V. (2022). SARS-CoV-2 mRNA-vaccine candidate; COReNAPCIN®, induces robust humoral and cellular immunity in mice and non-human primates. NPJ Vaccines, 7, 105.
+ Open protocol
+ Expand
8

Flow Cytometry Antibody Labeling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sheep anti-human C3c-FITC was obtained from AbD Serotec (cat. # AHP031F), anti-mouse IgG FITC and anti-human IgG FITC were from Sigma. Both antibodies (Abs) were used at a dilution of 1:200 in HBSS++ and 1% bovine serum albumin (BSA) (HBSS++/BSA) in flow cytometry assays.
Shaughnessy J., Gulati S., Agarwal S., Unemo M., Ohnishi M., Su X.H., Monks B.G., Visintin A., Madico G., Lewis L.A., Golenbock D.T., Reed G.W., Rice P.A, & Ram S. (2016). A novel factor H-Fc chimeric immunotherapeutic molecule against Neisseria gonorrhoeae. Journal of immunology (Baltimore, Md. : 1950), 196(4), 1732-1740.
+ Open protocol
+ Expand
9

Flow Cytometry and Western Blot Antibody Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-human IgG–FITC was from Sigma-Aldrich and was used at a dilution of 1:100 in HBSS containing 0.15 mM CaCl2 and 1 mM MgCl2 (HBSS++) and 1% BSA (HBSS++/BSA) in flow cytometry assays. Goat anti-human FH, alkaline phosphatase conjugated anti-human IgG (Southern Biotechnology), and donkey anti-goat IgG were used in Western blots a dilution of 1:1,000 in PBS with 5% non-fat dry milk.
Shaughnessy J., Tran Y., Zheng B., DeOliveira R.B., Gulati S., Song W.C., Maclean J.M., Wycoff K.L, & Ram S. (2020). Development of Complement Factor H–Based Immunotherapeutic Molecules in Tobacco Plants Against Multidrug-Resistant Neisseria gonorrhoeae. Frontiers in Immunology, 11, 583305.
+ Open protocol
+ Expand
10

Multiscreen Assay for Antigen-Specific IgG/IgA

Check if the same lab product or an alternative is used in the 5 most similar protocols
MultiscreenHTS HA plates (Millipore, MSHAN4510) were coated with 5μg/mL F protein antigen (Sino Biological Inc), 10μg/mL Human Serum Albumin (HSA, Sigma), 5μg/mL tetanus toxoid protein (Statens Seruminstitute) and 10μg/mL polyvalent goat anti-human immunoglobulins (Caltag). After washing, plates were blocked with 1% skimmed milk for 45 minutes at 37°C before 100μL/well PBMCs in R10 (RPMI, 10% Foetal Bovine Serum, 2mM L-Glutamine, 50μg/ml Streptomycin, 50U Penicillin) were added at a starting dilution of 2×106/mL and incubated overnight at 37°C, 5% CO2, 95% humidity. After washing plates were developed anti-human IgG-FITC (Sigma) and anti-human IgA-Biotin (AbD Serotec). After washing anti-FITC AP (Sigma) and Streptavidin-HRP (AbD Serotec) were added for 30 minutes at room temperature. Following final washes 3-Amino-9-ethylcarbazole (AEC) substrate kit (Sigma) was added for 30 minutes at room temperature, washed with dH2O and 100μL/well Vector Blue substrate (Vector Laboratories) added for 10 minutes at room temperature before a final wash in dH2O. After drying overnight plates were read using Autoimmun Diagnostika (AID version 5.0) and responses measured as the antigen-specific spots per million PBMCs with HSA background subtracted. A positive response was defined as any detection of spots above HSA background.
Green C., Scarselli E., Sande C., Thompson A., de Lara C., Taylor K., Haworth K., Del Sorbo M., Angus B., Siani L., Di Marco S., Traboni C., Folgori A., Colloca S., Capone S., Vitelli A., Cortese R., Klenerman P., Nicosia A, & Pollard A. (2015). Chimpanzee adenovirus and MVA-vectored respiratory syncytial virus vaccine is safe and expands humoral and cellular immunity in adults. Science translational medicine, 7(300), 300ra126.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!