Td 03314

Manufactured by Inotiv

The TD 03314 is a laboratory device designed for use in various research and analytical applications. It provides a core function of accurately measuring and monitoring specific parameters within a controlled environment. The detailed technical specifications and intended applications of this product are not available.

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Lab products found in correlation

D12492, by Research Diets (1 mentions) Teklad global 18 protein rodent diet 2918, by Inotiv (1 mentions) Adiponectin cre transgenic mice, by Jackson ImmunoResearch (1 mentions) Td 06414, by Inotiv (1 mentions) Crispr cas mediated genome engineering, by Cyagen (1 mentions)

3 protocols using td 03314

Metabolic Phenotyping of Cyp8b1 and GPR119 KO Mice

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Cyp8b1^–/– and GPR119^–/– mice are on the C57BL/6 genetic background (Taconic #11784 and #TF1293). We crossed Cyp8b1^–/– mice with GPR119^–/– mice to obtain double knockout mice, and used littermate male mice between 8 and 15 weeks of age for experiments. Mice were fed a normal chow diet (3.4 kcal/g, Purina 5053, 24.7% kcal from protein, 62.1% carbohydrate and 13.2% fat), a fat-free diet (3.3 kcal/g, Envigo TD 03314, 24.2% kcal from protein and 75.8% from carbohydrate) or a high-fat diet (5.21 kcal/g, Research Diets D12492, 20.0% kcal from protein, 20.0% carbohydrate and 60.0% fat). Mice were provided with the diet and water ad libitum and maintained on a 12-hour light/dark cycle, set with lights on at 7:00. For food intake measurements, mice were individually housed, and the food dispenser was located inside the cage.
Detailed methods can be found in supplementary materials and methods.

Higuchi S., Ahmad T.R., Argueta D.A., Perez P.A., Zhao C., Schwartz G.J., DiPatrizio N.V, & Haeusler R.A. (2020). Bile acid composition regulates GPR119-dependent intestinal lipid sensing and food intake regulation in mice. Gut, 69(9), 1620-1628.

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Metabolic Phenotyping of Knockout Mice

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Cyp8b1^−/− and GPR119^−/−mice are on the C57BL/6 genetic background (Taconic #11784 and #TF1293). We crossed Cyp8b1^−/− mice with GPR119^−/− mice to obtain double knock out mice, and used littermate male mice between 8-15 weeks of age for experiments. Mice were fed a normal chow diet (3.4 kcal/g, Purina 5053, 24.7% kcal from protein, 62.1% carbohydrate and 13.2% fat), a fat-free diet (3.3 kcal/g, Envigo TD 03314, 24.2% kcal from protein and 75.8% from carbohydrate) or a high-fat diet (5.21 kcal/g, Research diets D12492, 20.0% kcal from protein, 20.0% carbohydrate and 60.0% fat). Mice were provided with the diet and water ad libitum and maintained on a 12-hour light/dark cycle, set with lights on at 7 AM. For food intake measurements, mice were individually housed, and the food dispenser was located inside the cage. All experiments were approved and conducted according to the guidance of the Columbia University Institutional Animal Care and Use Committee.
Detailed methods can be found in the supplementary materials and methods.

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Adipocyte IRAKM Knockout Mouse Model

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IRAKM knockout mice were generated by Dr. Richard Flavell (Yale School of Medicine, New Haven) as described^{51 (link)}. IRAKM flox/flox mice were generated by our lab as described^{52 (link)}. Adipocyte-specific deletion of IRAKM (IRAKM^AKO) was obtained by breeding IRAKM flox/flox mice with Adiponectin-Cre transgenic mice (Jackson Laboratory, 028020). IRAKM kinase-dead(K205A) knock-in mice were generated by CRISPR/Cas-mediated genome engineering (Cyagen Biosciences). Six- to eight-week-old male mice were maintained on a high-fat diet composed of 60% kcal derived from fat (TD06414, Envigo) for 12 weeks, or on high carbohydrate (62% Sucrose)/zero-fat diet (TD03314, Envigo) for 8 weeks while normal mice maintained on either standard rodent chow diet (2918 Teklad Global 18% Protein Rodent Diet, Envigo). Animals were housed in a specific pathogen-free facility at a temperature of 21 °C, relative humidity of 50–70%, and under a constant 12-h light/dark cycle and given free access to food and water. All procedures using animals were approved by the Institutional Animal Care and Use Committee (IACUC) of Cleveland Clinic (protocol number: 2020–2316). Ethical compliance with IACUC protocols and institute standards was maintained.

Liu W., Zhou H., Wang H., Zhang Q., Zhang R., Willard B., Liu C., Kang Z., Li X, & Li X. (2022). IL-1R-IRAKM-Slc25a1 signaling axis reprograms lipogenesis in adipocytes to promote diet-induced obesity in mice. Nature Communications, 13, 2748.

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