Log In Sign up
The largest database of trusted experimental protocols

Stat4 clone c46b10

Manufactured by Santa Cruz Biotechnology
Visit Supplier

STAT4 clone (C46B10) is a mouse monoclonal antibody that recognizes the STAT4 protein. STAT4 is a transcription factor that plays a crucial role in the differentiation and function of T helper 1 (Th1) cells. The antibody can be used for various applications, such as Western blotting, immunoprecipitation, and flow cytometry, to detect and analyze the STAT4 protein in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

β actin clone c4, by Santa Cruz Biotechnology (2 mentions) Zeb1 clone e2g6y, by Santa Cruz Biotechnology (2 mentions) Jak2 clone d2e12, by Santa Cruz Biotechnology (2 mentions) Stat3 clone 124h6, by Santa Cruz Biotechnology (2 mentions) Pstat3 y705 clone d3a7, by Santa Cruz Biotechnology (2 mentions) Pstat3 s727 clone d8c2z, by Santa Cruz Biotechnology (2 mentions) Tyk2 clone d4i5t, by Santa Cruz Biotechnology (2 mentions)

Spelling variants of this product

STAT4 (5 citations)

2 protocols using stat4 clone c46b10

1

Immunoblotting Analysis of Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were harvested and lysed. Protein concentration was determined by BCA (Cat: #23227, Pierce) and 10 ug of protein were boiled in SDS containing Laemmli buffer. Protein was loaded on SDS-PAGE gel, and immunoblotting was performed using standard protocols. Antibodies were from cell signaling technology unless otherwise noted and included ZEB1 (clone E2G6Y), JAK2 (clone D2E12), STAT3 (clone 124H6), pSTAT3 Y705 (clone D3A7), pSTAT3 S727 (clone D8C2Z), STAT4 clone (C46B10), pSTAT4 Y693 (clone D2E4), JAK1 (clone D1T6W), JAK3 (clone D7B12), TYK2 (clone D4I5T), and β-actin (clone C4, Santa Cruz Biotechnology).
Qian Y., Arellano G., Ifergan I., Lin J., Snowden C., Kim T., Thomas J.J., Law C., Guan T., Balabanov R.D., Kaech S.M., Miller S.D, & Choi J. (2021). ZEB1 promotes pathogenic Th1 and Th17 cell differentiation in multiple sclerosis. Cell reports, 36(8), 109602.
+ Open protocol
+ Expand
2

Immunoblotting Analysis of Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were harvested and lysed. Protein concentration was determined by BCA (Cat: #23227, Pierce) and 10 ug of protein were boiled in SDS containing Laemmli buffer. Protein was loaded on SDS-PAGE gel, and immunoblotting was performed using standard protocols. Antibodies were from cell signaling technology unless otherwise noted and included ZEB1 (clone E2G6Y), JAK2 (clone D2E12), STAT3 (clone 124H6), pSTAT3 Y705 (clone D3A7), pSTAT3 S727 (clone D8C2Z), STAT4 clone (C46B10), pSTAT4 Y693 (clone D2E4), JAK1 (clone D1T6W), JAK3 (clone D7B12), TYK2 (clone D4I5T), and β-actin (clone C4, Santa Cruz Biotechnology).
Qian Y., Arellano G., Ifergan I., Lin J., Snowden C., Kim T., Thomas J.J., Law C., Guan T., Balabanov R.D., Kaech S.M., Miller S.D, & Choi J. (2021). ZEB1 promotes pathogenic Th1 and Th17 cell differentiation in multiple sclerosis. Cell reports, 36(8), 109602.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!