G-AgNPs and G-AuNPs, as well as the surface morphology of the SPCNFE electrodes, were characterized using a JEM-1400 transmission electron microscope (TEM) from JEOL (Tokyo, Japan) and a Gemini scanning electron microscope (SEM) from ZEISS® (Jena, Germany). TEM and STEM images were used to determine the size distribution of the obtained G-AgNPs and G-AuNPs. The size distribution histograms were calculated using Image-J version 1.51m software (National Institutes of Health (NIH, Bethesda, MD, USA).
Gemini scanning electron microscope
Manufactured by Zeiss
Sourced in Japan, Germany
The Gemini scanning electron microscope is a high-performance imaging tool designed for researchers and industry professionals. It captures detailed, high-resolution images of samples by scanning them with a focused beam of electrons. The microscope's core function is to provide a comprehensive view of the surface structure and composition of materials at the nanoscale level.
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5 protocols using gemini scanning electron microscope
1
Characterization of G-AgNPs and G-AuNPs
2
Microstructural Analysis by Scanning Electron Microscopy
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Following microcomputed tomography to confirm proper orientation of region of interest, specimens were mounted on aluminum pins with conductive silver epoxy (Ted Pella, Redding, CA). The specimens were trimmed to remove excess resin above ROI and to remove silver epoxy from sides of specimen. The specimens were sputter coated with gold-palladium and then imaged using a Gemini scanning electron microscope (Zeiss) equipped with a 3View2XP and OnPoint backscatter detector (Gatan). Images were acquired at 2.5 kV accelerating voltage with a 30 μm condenser aperture and 1 μsec dwell time; Z step size was 50 nm; raster size was 12k × 9k and Z dimension was 1200 sections. Volumes were either collected in variable pressure mode with a chamber pressure of 30 Pa and a pixel size of 3.8 nm (Figure 2—video 1 and Figure 3D ) or using local gas injection (Deerinck et al., 2018 (link)) set to 85% and a pixel size of 6.5 nm (Figures 2A, B and 3C ). Volumes were aligned using cross correlation, segmented, and visualized using IMOD (Kremer et al., 1996 (link)).
3
Morphological and Compositional Analysis of ZnS QDs
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The surface morphology and EDAX studies of ZnS QDs and ZnS QD-MPA are carried out using a ZEISS Gemini Scanning Electron Microscope. The sample preparation for this study is as detailed above in X-ray characterisation. The size and shape of the QDs are determined. EDAX gives information on the chemical composition of the sample. Using the weight percentage obtained from the results, the MPA to ZnS ratio was calculated.
4
Characterization of Dynabeads-COOH by Imaging Flow Cytometry and SEM
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Commercial Dynabeads-COOH (InvitrogenTM, Waltham, MA, USA) were purchased from Thermofisher Scientific. For characterization of their size, morphology, and concentration by imaging flow cytometry, the microbeads were diluted 1/100 in 10 mM phosphate-buffered saline (PBS) with pH of 7.20 at 40 mm/s, and each event was photographed at 60× magnification using ImageStream equipment. The IDEAS software was used for the data analysis. For characterization by SEM, the microbeads were fixed in a solution of PBS with 4% paraformaldehyde and 1% glutaraldehyde for 24 h and were then washed in PBS followed by incubation in 2% osmium tetroxide for 1 h. Microbeads were then fully dehydrated in serial ethanol solutions. Finally, the dry sample was sprinkled onto slide glass and metalized for photography with the Zeiss Gemini scanning electron microscope.
5
Characterization of Ag Nanoparticles on SPCNFE
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Colloid solutions of Ag-NS and Ag-NPr nanoparticles were characterized by using a Gemini scanning electron microscope from ZEISS® (Jena, Germany). Ag-NP samples were prepared as in a previous work [28 (link)]. In addition, the surface of the SPCNFE electrodes was also studied by the scanning electron microscope before and after the Ag-NP deposition. This aimed to determine the presence of NPs, as well as their spatial distribution on the SPCNFE with regard to the modification strategy: drop-casting, spin-coating, or in situ.
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