Ha vsmcs

Manufactured by ScienCell

Sourced in United States

HA-VSMCs are primary human aortic vascular smooth muscle cells. They are isolated from the human aorta and are cryopreserved at the end of the primary culture. HA-VSMCs retain their normal morphology and functionality.

Automatically generated - may contain errors

Lab products found in correlation

Inorganic phosphate, by Merck Group (1 mentions) Trypsin, by Merck Group (1 mentions) Penicillin streptomycin, by ScienCell (1 mentions) Growth supplement, by ScienCell (1 mentions) Smc medium, by ScienCell (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Ha vsmc, by American Type Culture Collection (1 mentions)

4 protocols using ha vsmcs

Modulation of VSMC Calcification by Exosomes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human aortic vascular smooth muscle cells (HA-VSMCs, ScienCell, USA) were cultured in SMC medium (ScienCell, USA) supplemented with 2% FBS. Human BMSCs were cultured in MSC medium supplemented with 5% exosome-depleted FBS (ScienCell, USA).
To induce calcification, HA-VSMCs were treated with 2.5 mmol/L Pi for 14 days. To observe the contribution of exosomes and NONHSAT 084969.2 on calcification, HA-VSMCs were treated with 100 μg/ml BMSC-Exos or small interfering RNA NONHSAT 084969.2 (si-NONHSAT 084969.2) for 14 days. Finally, calcification and osteogenic transdifferentiation were evaluated.

Liu Y., Bao S., Guo W, & Liu W. (2021). Bone mesenchymal stem cell derived exosomes alleviate high phosphorus-induced calcification of vascular smooth muscle cells through the NONHSAT 084969.2/NF-κB axis. Aging (Albany NY), 13(12), 16749-16762.

+ Open protocol

+ Expand

Inorganic Phosphate-Induced VSMC Calcification

Check if the same lab product or an alternative is used in the 5 most similar protocols

HA-VSMCs were obtained from ScienCell Research Laboratories (CA, USA), and were cultured in SMC medium containing 2% fetal bovine serum (FBS). HA-VSMCs were stimulated with inorganic phosphate (final concentration 2.5 mM, pH 7.4) (Sigma-Aldrich, MI, USA) to induce calcification [41 (link)]. Cells in our experiments were all tested for short tandem repeat analysis and were free of mycoplasma contamination.

Liu Y., Guo Y., Bao S., Huang H., Liu W, & Guo W. (2022). Bone marrow mesenchymal stem cell-derived exosomal microRNA-381-3p alleviates vascular calcification in chronic kidney disease by targeting NFAT5. Cell Death & Disease, 13(3), 278.

+ Open protocol

+ Expand

Aortic Smooth Muscle Cell Calcification

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human aortic smooth muscle cells (HA-VSMCs) was purchased from ScienCell (USA). An VSMC culture medium containing 10% fetal bovine serum, 1% penicillin-streptomycin and 1% growth supplement (ScienCell, USA) was cultured in incubators with 5% CO₂ at 37 °C. Once cells had grown to 90% confluence in the Petri dish, 0.25% trypsin (Sigma, USA) was added for harvesting. The passage ratio was 1:3. HA-VSMCs from the fourth to sixth passage were used in subsequent experiments. The cells were cultured in Dulbecco’s Modified Eagle’s Medium (contained 2.5 mM Na₂HPO₄) for 7 days to induce calcification. The medium was replaced every other day.

Bao S., Guo Y., Diao Z., Guo W, & Liu W. (2020). Genome-wide identification of lncRNAs and mRNAs differentially expressed in human vascular smooth muscle cells stimulated by high phosphorus. Renal Failure, 42(1), 437-446.

+ Open protocol

+ Expand

HA-VSMC Culture and Oxidized LDL Exposure

Check if the same lab product or an alternative is used in the 5 most similar protocols

HA-VSMCs were provided by the American Type Culture Collection (ATCC; Rockville, MD, USA). HA-VSMCs were maintained in SMC medium (SMCM; ScienCell, Carlsbad, CA, USA) and cultured in an incubator containing 5% CO₂ at 37 ℃. HA-VSMCs were passaged every 3–4 days. The medium was added with 1% penicillin-streptomycin and 2% fetal bovine serum (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). HA-VSMCs were incubated for 24 h. Oxidized LDL (ox-LDL) was prepared at concentrations of 25, 50, 75, and 100 µg/mL.

Song Z., Han Q., Wen Z., Lv Q., Pan C, & Pan Y. (2023). LncRNA RASSF8-AS1 knockdown displayed antiproliferative and proapoptotic effects through miR-188-3p/ATG7 pathway in ox-LDL-treated vascular smooth muscle cells. Annals of Translational Medicine, 11(3), 143.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!