Mouse (Catalog I9646) and human (Catalog I1395) IL-6 were from MilliporeSigma. Mouse (Catalog 713704) and human (Catalog 713604) GM-CSF and mouse (Catalog 713502) and human (Catalog 713402) G-CSF were purchased from Biolegend. The T cell proliferation dye used was CellTrace Calcein Violet (ThermoFisher Scientific, Catalog C34858). propranolol (Catalog P0884) and isoproterenol (Catalog I6504) were purchased from MilliporeSigma. In vitro, propranolol was used at a concentration of 1 μM and isoproterenol was used at a concentration of 10 μM. Etomoxir (Tocris, Catalog 4539) was used in vivo at a dose of 10mg/kg and in vitro at a concentration of 10 μM. To stain MDSCs for autophagic vesicles, the CYTO-ID® Autophagy detection kit 2.0 (Catalog ENZ-51031-0050) from Enzo Life Sciences was used. LPS (MilliporeSigma, Catalog L3024) was used at a concentration of 10ng/ml. Torin-1 (Cell Signaling, Catalog 14379S) was used at a concentration of 250 nM. 3-MA (Torics, Catalog 3977) was used at a concentration of 2 mM. Chloroquine (Enzo Life Sciences, Catalog 51031) was used at a concentration of 10 μM.
Mouse catalog 713704 and human catalog 713604 gm csf
Manufactured by BioLegend
Mouse GM-CSF (Catalog 713704) and human GM-CSF (Catalog 713604) are recombinant cytokines produced in E. coli. GM-CSF stands for Granulocyte-Macrophage Colony-Stimulating Factor, which is a protein that regulates the production and function of granulocytes and macrophages.
Automatically generated - may contain errors
Lab products found in correlation
Celltrace calcein violet, by Thermo Fisher Scientific (2 mentions)
Mouse catalog i9646, by Merck Group (2 mentions)
Human catalog i1395 il 6, by Merck Group (2 mentions)
Propranolol catalog p0884, by Merck Group (2 mentions)
Isoproterenol catalog i6504, by Merck Group (2 mentions)
Propranolol, by Bio-Techne (2 mentions)
Chloroquine, by Enzo Life Sciences (2 mentions)
Isoproterenol, by Bio-Techne (2 mentions)
Cyto id autophagy detection kit 2, by Enzo Life Sciences (2 mentions)
Etomoxir, by Bio-Techne (2 mentions)
Mouse catalog 713502 and human catalog 713402 g csf, by BioLegend (2 mentions)
Torin 1, by Cell Signaling Technology (2 mentions)
2 protocols using mouse catalog 713704 and human catalog 713604 gm csf
1
Investigating Immune Modulation in Preclinical Models
2
Investigating Immune Modulation in Preclinical Models
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Mouse (Catalog I9646) and human (Catalog I1395) IL-6 were from MilliporeSigma. Mouse (Catalog 713704) and human (Catalog 713604) GM-CSF and mouse (Catalog 713502) and human (Catalog 713402) G-CSF were purchased from Biolegend. The T cell proliferation dye used was CellTrace Calcein Violet (ThermoFisher Scientific, Catalog C34858). propranolol (Catalog P0884) and isoproterenol (Catalog I6504) were purchased from MilliporeSigma. In vitro, propranolol was used at a concentration of 1 μM and isoproterenol was used at a concentration of 10 μM. Etomoxir (Tocris, Catalog 4539) was used in vivo at a dose of 10mg/kg and in vitro at a concentration of 10 μM. To stain MDSCs for autophagic vesicles, the CYTO-ID® Autophagy detection kit 2.0 (Catalog ENZ-51031-0050) from Enzo Life Sciences was used. LPS (MilliporeSigma, Catalog L3024) was used at a concentration of 10ng/ml. Torin-1 (Cell Signaling, Catalog 14379S) was used at a concentration of 250 nM. 3-MA (Torics, Catalog 3977) was used at a concentration of 2 mM. Chloroquine (Enzo Life Sciences, Catalog 51031) was used at a concentration of 10 μM.
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