B6.129x1 trpv1tm1jul

All experiments were approved by the Berlin animal ethics committee and carried out in accordance with European animal welfare law. Adult Wild-type C57Bl6/J mice and transgenic mice were used. Both male and female mice were used in this study, but no obvious differences were observed between sexes. All mice were given ad libitum access to food and water, except in for prior to behavioral testing (see below). The following strains of transgenic mice were used: 1) trpv1^−/− mice on a mixed background, from Jackson Laboratories (B6.129X1-Trpv1^tm1Jul) (Caterina et al., 2000 (link)). 2) Trpm2^−/− mice on a mixed background (129/SvJ and C57Bl6/N), backcrossed with C57Bl6/J mice for several generations, kindly donated by Yasuo Mori, Kyoto University (Yamamoto et al., 2008). 3) Trpm8^−/− mice on a mixed background, from Jackson Laboratories (B6.129P2-Trpm8^tm1Jul) (Bautista et al., 2007 (link)). 4). The trpv1:trpa1:trpm3^−/− triple knockout mice on a C57BL/6J background were generated by Thomas Voets and Joris Vriens and made available for this study (Vandewauw et al., 2018 (link)). All mice were maintained on a 12h light/ 12h dark cycle.