Human and mouse fgf21 elisa kits

β-OHB and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). Entinostat was obtained from Selleck Chemicals (Houston, TX, USA). Dulbecco’s modified eagle medium (DMEM), fetal bovine serum (FBS), and the other reagents for cell culture were the products of Thermo Fisher (Waltham, MA, USA). The kits for RNA extraction, reverse transcription (RT), quantitative PCR (qPCR) and the kits for cellular protein extraction and measurement were obtained from Takara Bio USA Inc (Mountain View, CA, USA). HDAC activity colorimetric assay kits were the products of BioVision Incorporated (Milpitas, CA, USA). Histone H3ac (pan-acetyl) polyclonal antibody, glyceraldehyde phosphate dehydrogenase (GAPDH) polyclonal antibody, horseradish peroxidase (HRP)-conjugated goat-anti rabbit antibody, and 20× Tris-buffered solution (TBS) were purchased from Thermo Fisher. Human and mouse FGF21 ELISA kits, glutamic–pyruvic transaminase (ALT) activity assay kit, and glutamic–oxaloacetic transaminase (AST) activity assay kit were the products of Elabscience (Wuhan, China). Hematoxylin–eosin (H&E) staining kits were obtained from Beyotime Biotechnology (Beijing, China).

Pyruvate, Compound C, GW6471, forskolin, IBMX, 8-Bromo-cAMP, H89, ESI-09, 666-15 and MTT were purchased from Merck KGaA (Darmstadt, Germany). Dulbecco’s modified eagle medium (DMEM), fetal bovine serum (FBS) and trypsin for cell culture were obtained from Thermo Fisher (Waltham, MA, USA). The kits for RNA extractive, reverse transcription (RT) and quantitative PCR (qPCR) were obtained from Takara Bio Inc (Dalian, China). Protein extraction kits and BCA assay kits were purchased from Bio-Rad Laboratories (Hercules, USA). Human and mouse FGF21 ELISA kits, cAMP assay kits, the ALT activity assay kit, the AST activity assay kit and the LDH assay kit were obtained from Elabscience (Wuhan, China). Pyruvate assay kits were obtained from Abcam (Boston, MA, USA). Anti-cAMP response element-binding protein (CREB) and anti-phospho-CREB (Ser133) antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA). H&E staining kits were obtained from Beyotime Biotechnology (Beijing, China).