2996 uv detector

Manufactured by Waters Corporation

Sourced in United States

The 2996 UV Detector is a high-performance liquid chromatography (HPLC) detector that measures the ultraviolet (UV) absorbance of analytes as they elute from the HPLC column. It is designed to provide accurate and reliable detection of a wide range of organic compounds.

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Lab products found in correlation

Millennium 32, by Waters Corporation (2 mentions) Acetonitrile, by Merck Group (1 mentions) Waters alliance 2695 separation module, by Waters Corporation (1 mentions) Genesys 20, by Thermo Fisher Scientific (1 mentions) 2410 refractive index detector, by Waters Corporation (1 mentions)

Spelling variants of this product

UV detector (22 citations) 2996 Photodiode Array UV detector (3 citations) Waters 2996 UV detector (2 citations) PDA 2996 UV detector (2 citations)

5 protocols using 2996 uv detector

Characterization of Gold Nanoparticles

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The composition of EAR was identified using a Waters 2695 high performance liquid system (HPLC), including 717 auto-sampler manager and 2996 UV detector, connected to Millennium 32 software (Waters, USA). HPLC conditions were as follows: a Spursil C18 column (250 × 4.6 mm, 5 μm) was used. The mobile phase was formed by solvent A (acetonitrile) and solvent B (water solution) with a linear gradient as follows: 0–30 min: 30% A, 30–65 min: 60% A. The flow rate was 1.0 mL min⁻¹, column temperature was 30 °C and injection volume was 20 μL. The spectrum was monitored at 280 nm.
Fourier transform infrared (FT-IR) spectra were conducted on a Nicolet NEXUS-870 FT-IR spectrometer (Thermo Nicolet, USA). An ultraviolet spectrometer (UV-vis, Perkin Elmer, USA) equipped with a 1 cm-path-length quartz cell was operated to obtain the UV-vis spectrum. The size of AuNPs was confirmed on the laser dynamic scattering instrument Zetasizer Nano 3600 (Malvern, UK). Transmission electron microscopy (TEM) images were measured on an FEI Tecnai G2TF20 instrument (FEITECNAI, USA) operating at an acceleration voltage of 200 kV. The laser dynamic scattering instrument, Zetasizer Nano 3600 (DLS, Malvern, UK), was used to record the size of nanoparticles in the solution.

Chen X., Ji J., Shi G., Xue Z., Zhou X., Zhao L, & Feng S. (2020). Formononetin in Radix Hedysari extract-mediated green synthesis of gold nanoparticles for colorimetric detection of ferrous ions in tap water. RSC Advances, 10(54), 32897-32905.

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HPLC Analysis of Freeze-Dried GBH Sample

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The eluent solvents used consisted of A: water (10 mM KH₂PO₄ solution at pH 4.5), and B: acetonitrile. We used an X Bridge BEH C18 HPLC column (130 Å, 5 µm and 4.6 mm × 250 mm; Waters, Milford, MA, USA). Gradient: 0–15 min: 5% B, 50 min: 70% B, 65 min: 100% B, 65–75 min: 100% B. Flow rate: 0.9 mL/min. Column temperature was maintained at 40 °C. Sample concentration: 50 mg/mL (dissolving solvent 60% ethanol, sonication time—1 h). The injection volume was 10 μL. The standard chemical concentration varied from 1 to 150 μg/mL with similar injection volume of 10 μL. Based on the standard compounds’ concentrations and their respective signal intensity correlations, we established a specific regression equation for each compound to confirm a good linear range and to calculate their concentration in the freeze-dried GBH sample. Analysis was carried out using a Waters HPLC 2965 separation module and a Waters 2996 UV detector.

Yang J.H., Nguyen C.D., Lee G, & Na C.S. (2022). Insamgobonhwan Protects Neuronal Cells from Lipid ROS and Improves Deficient Cognitive Function. Antioxidants, 11(2), 295.

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Quantification of Caffeine and 5-CA

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Quantification of caffeine and 5-CA was carried out using a high-performance liquid chromatograph (HPLC) coupled to a Waters 2996 UV Detector (Milford, MA, USA). The stationary phase was an Ultra C8 column (5 µm particle size, 150 mm length, 4.6 mm i.d.) from Scharlab (Barcelona, Spain). All data were processed using the Empower Solutions Software (Orlando, FL, USA).
A Velp Scientifica F202A0176 vortex shaker (Monza et de la Brianza, Italy), a J.P. Selecta 7002575 centrifuge, and a 3000865 ultrasound bath (Barcelona, Spain) were used for sample preparation.

Bouhzam I., Cantero R., Balcells M., Margallo M., Aldaco R., Bala A., Fullana-i-Palmer P, & Puig R. (2023). Environmental and Yield Comparison of Quick Extraction Methods for Caffeine and Chlorogenic Acid from Spent Coffee Grounds. Foods, 12(4), 779.

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HPLC Fingerprinting and Bone Analyses of Radix Hedysari

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The HPLC fingerprints of Radix Hedysari were performed using a Waters 2695 high performance liquid system, including 717 auto-sampler manager and 2996 UV detector, connected to Millennium 32 software (Waters, USA). Dual energy X-ray absorptiometry (GE Prodigy, USA) was used to measure bone mineral density (BMD) rapidly and accurately. AG-X desktop electronic universal test machine (Shimadzu, Japan) was used to measure bone biomechanics as a convenient tool. Microplate reader (Bio-Rad 550, USA) was used to determine the spectral absorbance of samples.
Acetonitrile and pentyl barbital sodium were of analytical grade (Merck, Germany). Purified water was purchased from Wahaha Company (China). Reference standards of adenosine, calycosin-7-glucoside, ononin, calycosin, and formononetin were purchased from China drugs and biological products assay (Beijing, China, purity > 98%). Alkaline phosphatase (AKP), tartrate resistant acid phosphatase (TRACP), calcium (Ca), and phosphorus (P) were purchased from Jiancheng Biopharmaceutical Company (Nanjing, China).

Chen X.Y., Gou S.H., Shi Z.Q., Xue Z.Y, & Feng S.L. (2018). Spectrum-effect relationship between HPLC fingerprints and bioactive components of Radix Hedysari on increasing the peak bone mass of rat. Journal of Pharmaceutical Analysis, 9(4), 266-273.

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Quantifying Bacterial Growth Metrics

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Cell density was determined by plating onto MRS agar at 37°C for 48 h. Cell density was also monitored using either optical density at 620 nm (OD 620 ) with a Genesys 20 spectrophotometer (Thermo Fisher Scientific Inc., Waltham, MA, USA), or cell dry weight (CDW). For CDW determination, a known volume of fermentation broth was centrifuged for 10 min at 4°C and 3,000 ×g in pre-weighed tubes, washed once with water, and dried into a microwave oven at 600 W until stable weight was reached. CDW was routinely determined using a standard curve relating OD 620 values to CDW; the correlation factor was CDW (g/l) = 0.43 × OD 620 .
Concentrations of glucose, acetic and lactic acids, glycerol, 1,3-PDO, and ethanol were determined by HPLC analysis using a Waters Alliance 2695 separation module (Waters, Milford, MA, USA) equipped with a Rezex ROA-Organic Acid H+ (8%) 300 mm × 7.8 mm column (Phenomenex Inc., USA), coupled to a Waters 2410 refractive index detector and a Waters 2996 UV detector. Separation was carried out at 65°C with 0.005 M H 2 SO 4 as the mobile phase at a flow rate of 0.6 ml/min.

Ricci M.A., Russo A., Pisano I., Palmieri L., de Angelis M, & Agrimi G. (2015). Improved 1,3-Propanediol Synthesis from Glycerol by the Robust Lactobacillus reuteri Strain DSM 20016. Journal of microbiology and biotechnology, 25(6).

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