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Phospho stat6

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Phospho-STAT6 is a lab equipment product that detects and quantifies the phosphorylated form of the STAT6 protein. STAT6 is a transcription factor involved in the signaling pathways of various cytokines. The phosphorylated form of STAT6 is important for its activation and subsequent regulation of gene expression.

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Lab products found in correlation

Lsr 2 flow cytometer, by BD (2 mentions) Cd206 apc, by BioLegend (2 mentions) Cd11b pe cy7, by BioLegend (2 mentions) Cd11c apc cy7, by BioLegend (2 mentions) F4 80 af488, by BioLegend (2 mentions) Phospho stat1, by BioLegend (2 mentions) Gr 1 pe, by BioLegend (2 mentions) Supersignal west femto maximum sensitivity substrate, by Thermo Fisher Scientific (1 mentions) α tubulin, by Cell Signaling Technology (1 mentions) Nupage gel, by Thermo Fisher Scientific (1 mentions) Chemidoc imaging system, by Bio-Rad (1 mentions) Nitrocellulose membrane, by GE Healthcare (1 mentions) Amersham ecl western blotting reagent, by GE Healthcare (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Complete mini tablet, by Roche (1 mentions) Phosphostop, by Roche (1 mentions) Amersham ecl, by Cytiva (1 mentions)

3 protocols using phospho stat6

1

Flow Cytometry Analysis of Immune Cells

Cited 1 time
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Flow-cytometric analysis of at least 105 cells per sample was performed using a BD LSR II Flow Cytometer (BD Biosciences) following protocol as described in Dolgachev et al (2012) (link)17 and using the following antibodies Gr-1-PE,CD11c-APC-Cy7, F4/80-AF488, CD11b-PE-Cy7, CD206-APC, phospho-STAT1 and phospho-STAT6 (BioLegend and BD Biosciences, San Jose, CA, USA). Obtained data were plotted and analyzed using the FlowJo software (Tree Star, Inc., Ashland, OR, USA).
Dolgachev V., Panicker S., Balijepalli S., McCandless L.K., Yin Y., Swamy S., Suresh M., Delano M.J., Hemmila M.R., Raghavendran K, & Machado-Aranda D. (2018). Electroporation-mediated Delivery of FER Gene Enhances Innate Immune Response and Improves Survival in a Murine Model of Pneumonia. Gene therapy, 25(5), 359-375.
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2

Western Blot Analysis of Protein Targets

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For total protein extraction, cells were lysed in urea lysis buffer [8 M Urea, 0.5% Triton-X, cOmplete Tablet Mini, PhosphoStop (both Roche Diagnostics)]. Cellular fractionation lysates were prepared as described [20 (link)], complemented with longer centrifugation and washing of the nuclear pellet for higher purity. Proteins were separated on 4–12% NuPage Gels (Thermo Fisher Scientific) and transferred to nitrocellulose membranes (GE Healthcare Life Science). Primary antibodies against AID (#4949), β-actin (#4967), p65 (#4764), p100/p52 (#4882), RelB (#4922), αTubulin (#3873, all Cell Signaling Technologies), Smad2/3 (GTX111123), Stat6 (GTX113273, both GeneTex) and Phospho-Stat6 (686002, Biolegend®) were used. Primary antibodies were detected using HRP-labeled secondary antibodies (Cell Signaling Technologies) and Amersham ECL™ Western Blotting reagent (GE Healthcare Life Science) or Supersignal™ West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific) using a ChemiDoc Imaging System (Bio-Rad Laboratories).
Traxel S., Lehmann J., Richard S., Sidorov S., Niggli F., Berger C., Nadal D, & Bürgler S. (2021). Support of BCP-ALL-cells by autologous bone marrow Th-cells involves induction of AID expression but not widespread AID off-target mutagenesis. Cancer Immunology, Immunotherapy, 70(8), 2275-2289.
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3

Flow Cytometry Analysis of Immune Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Flow-cytometric analysis of at least 105 cells per sample was performed using a BD LSR II Flow Cytometer (BD Biosciences) following protocol as described in Dolgachev et al (2012) (link)17 and using the following antibodies Gr-1-PE,CD11c-APC-Cy7, F4/80-AF488, CD11b-PE-Cy7, CD206-APC, phospho-STAT1 and phospho-STAT6 (BioLegend and BD Biosciences, San Jose, CA, USA). Obtained data were plotted and analyzed using the FlowJo software (Tree Star, Inc., Ashland, OR, USA).
Dolgachev V., Panicker S., Balijepalli S., McCandless L.K., Yin Y., Swamy S., Suresh M., Delano M.J., Hemmila M.R., Raghavendran K, & Machado-Aranda D. (2018). Electroporation-mediated Delivery of FER Gene Enhances Innate Immune Response and Improves Survival in a Murine Model of Pneumonia. Gene therapy, 25(5), 359-375.
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