Cells were lysed in radioimmunoprecipitation assay (RIPA) lysis buffer (Beyotime) added with protease inhibitor (Selleck) and phosphatase inhibitor (Selleck). The extracted or enriched proteins were separated by 12% SDS-PAGE. After being transferred into polyvinylidene fluoride (PVDF) membrane (Millipore), the blots were incubated with primary antibodies against EZH2 (1:500; 07-689; Millipore), PCDH7 (1:200; ab139274; Abcam), phospho-ERK1/2 (1:1,000; #4370; Cell Signaling Technology), ERK1/2 (1:1,000; #4695; Cell Signaling Technology), phospho-c-FOS (1:1,000; #5348; Cell Signaling Technology), c-FOS (1:1,000; #2250; Cell Signaling Technology), or GAPDH (1:10,000; T0004; Affinity). After three washes, the blots were further incubated with goat anti-mouse immunoglobulin G (IgG) H&L (IRDye 680RD) preadsorbed secondary antibody (1:10,000; ab216776; Abcam) or goat anti-rabbit IgG H&L (IRDye 800CW) preadsorbed secondary antibody (1:10,000; ab216773; Abcam). The western blot images were obtained using Odyssey infrared scanner (Li-Cor).
Ab139274
Manufactured by Abcam
Sourced in United States
Anti-ACTR10 antibody (ab139274) is a rabbit polyclonal antibody that recognizes the ACTR10 protein. ACTR10 is a subunit of the dynactin complex, which is involved in the regulation of microtubule-based intracellular transport.
Automatically generated - may contain errors
Lab products found in correlation
Ab216776, by Abcam (1 mentions)
Ab216773, by Abcam (1 mentions)
Protease inhibitor, by Selleck Chemicals (1 mentions)
Phosphatase inhibitor, by Selleck Chemicals (1 mentions)
C fos, by Cell Signaling Technology (1 mentions)
Phospho c fos, by Cell Signaling Technology (1 mentions)
Irdye 800cw, by Abcam (1 mentions)
Irdye 680rd, by Abcam (1 mentions)
Phospho erk1 2, by Cell Signaling Technology (1 mentions)
Odyssey infrared scanner, by LI COR (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Erk1 2, by Cell Signaling Technology (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
Gapdh, by Affinity Biosciences (1 mentions)
Polyvinylidene difluoride membrane, by Merck Group (1 mentions)
2 protocols using ab139274
1
Protein Extraction and Western Blot Analysis
2
Immunoblotting of GAPDH and PCDH7
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total protein was extracted, quantified, and separated on 10% SDS-PAGE gels, and then transferred to polyvinylidene difluoride membranes (Millipore, MA, USA), which were incubated with primary anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Proteinch, 60004-1-Ig), anti-PCDH7 (Abcam, ab139274) primary antibodies at 4 °C overnight.
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