Log In Sign up
The largest database of trusted experimental protocols

Nicotine bitartrate dihydrate

Manufactured by MP Biomedicals
Visit Supplier
Sourced in United States

Nicotine bitartrate dihydrate is a chemical compound used in research and laboratory settings. It is a salt formed from the combination of nicotine and tartaric acid. The compound is a crystalline solid that is soluble in water and other polar solvents.

Automatically generated - may contain errors

Lab products found in correlation

Rolipram, by Bio-Techne (2 mentions) Atenolol, by Merck Group (2 mentions) Clozapine n oxide cno, by Enzo Life Sciences (2 mentions) Rp camps, by Bio-Techne (2 mentions) 8 br camp, by Bio-Techne (2 mentions) Insulin, by Merck Group (2 mentions) D glucose, by Merck Group (2 mentions) Glucagon, by Merck Group (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)

4 protocols using nicotine bitartrate dihydrate

1

Preparation of Solutions for Animal Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols
For self-administration experiments in mice and rats, nicotine bitartrate dihydrate (CAS# 6019–06-3, MP Biomedicals, Santa Ana, CA) was dissolved in 0.9% sterile saline. All doses of nicotine refer to the free-base form. Atenolol (CAS# 29122–68-7, Sigma-Aldrich, St. Louis, MO) was dissolved in 2mM HCl in 0.9% sterile saline. The GLP-1 receptor agonist Ex-4 (Tocris, Ellisville, MO) was dissolved in 0.9% saline solution for in vivo use and 0.1M PBS for ex vivo use. Clozapine-N-oxide (CNO, Enzo Life Sciences, Farmingdale, NY), insulin (CAS# 11070–73-8, Sigma-Aldrich, St. Louis, MO) and glucagon (CAS# 16941–32-5, Sigma-Aldrich, St. Louis, MO) were diluted in 0.9% saline for intraperitoneal injection. D-(+)-Glucose (CAS# 50–99-7, Sigma-Aldrich, St. Louis, MO) was dissolved in dH2O for oral gavage. cAMPS-RP, 8-Br-cAMP, and Rolipram (Tocris, Ellisville, MO) were all dissolved in 0.9% saline. The pH of all solutions was adjusted to ~7.4. The pH of all solutions was adjusted to ~7.4.
Duncan A., Heyer M.P., Ishikawa M., Caligiuri S.P., Liu X.A., Chen Z., di Bonaventura M.V., Elayouby K., Ables J.L., Howe W.M., Bali P., Fillinger C., Williams M., O’Connor R., Wang Z., Lu Q., Kamenecka T.M., Ma’ayan A., O’Neill H.C., Ibanez-Tallon I., Geurts A.M, & Kenny P.J. (2019). Habenular Tcf7l2 links nicotine addiction to diabetes. Nature, 574(7778), 372-377.
+ Open protocol
+ Expand
2

Menthol and Nicotine Pharmacological Effects

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
L-(−)-Menthol (Fisher Scientific, Atlanta, Georgia, USA) was dissolved in 50% dimethyl sulfoxide (DMSO; Sigma; Atlanta, Georgia, USA) and distilled water (v/v). Menthol and vehicle (50% DMSO: H2O, v/v) solutions were freshly prepared weekly and injected intraperitoneally (IP) at 1 ml/kg with a 5-min IPI. All menthol and vehicle solutions were heated to 50°C before each session to ensure menthol solubility. All nicotine and menthol training doses were selected based on previous literature (Biswas et al., 2016 (link); Huynh et al., 2019 (link)). (−)-Nicotine bitartrate dihydrate (MP Biomedicals; Solon, Ohio, USA) was dissolved in 0.9% saline, titrated with NaOH to a pH of 7.0 ± 0.2, and injected subcutaneously (SC) at 1 ml/kg with a 5-min IPI. All nicotine doses are expressed as the free base form and all menthol doses are expressed as the salt form.
Huynh Y.W., Raimondi A., Finkner A., Kuck J.D., Selleck C, & Bevins R.A. (2020). Menthol Blunts the Interoceptive Discriminative Stimulus Effects of Nicotine in Female but not Male Rats. Psychopharmacology, 237(8), 2395-2404.
+ Open protocol
+ Expand
3

Preparation of Solutions for Animal Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols
For self-administration experiments in mice and rats, nicotine bitartrate dihydrate (CAS# 6019–06-3, MP Biomedicals, Santa Ana, CA) was dissolved in 0.9% sterile saline. All doses of nicotine refer to the free-base form. Atenolol (CAS# 29122–68-7, Sigma-Aldrich, St. Louis, MO) was dissolved in 2mM HCl in 0.9% sterile saline. The GLP-1 receptor agonist Ex-4 (Tocris, Ellisville, MO) was dissolved in 0.9% saline solution for in vivo use and 0.1M PBS for ex vivo use. Clozapine-N-oxide (CNO, Enzo Life Sciences, Farmingdale, NY), insulin (CAS# 11070–73-8, Sigma-Aldrich, St. Louis, MO) and glucagon (CAS# 16941–32-5, Sigma-Aldrich, St. Louis, MO) were diluted in 0.9% saline for intraperitoneal injection. D-(+)-Glucose (CAS# 50–99-7, Sigma-Aldrich, St. Louis, MO) was dissolved in dH2O for oral gavage. cAMPS-RP, 8-Br-cAMP, and Rolipram (Tocris, Ellisville, MO) were all dissolved in 0.9% saline. The pH of all solutions was adjusted to ~7.4. The pH of all solutions was adjusted to ~7.4.
Duncan A., Heyer M.P., Ishikawa M., Caligiuri S.P., Liu X.A., Chen Z., di Bonaventura M.V., Elayouby K., Ables J.L., Howe W.M., Bali P., Fillinger C., Williams M., O’Connor R., Wang Z., Lu Q., Kamenecka T.M., Ma’ayan A., O’Neill H.C., Ibanez-Tallon I., Geurts A.M, & Kenny P.J. (2019). Habenular Tcf7l2 links nicotine addiction to diabetes. Nature, 574(7778), 372-377.
+ Open protocol
+ Expand
4

Nicotine Preparation and Shh Protein

Check if the same lab product or an alternative is used in the 5 most similar protocols
Nicotine bitartrate dihydrate (MP Biomedicals LLC, catalog no. 0521149910) was dissolved in saline and the pH adjusted to 7.6 with NaOH. Nicotine solutions were prepared daily in glass vials wrapped in aluminum foil to protect from light and sterilized by being passed through a 0.25-µm syringe filter (Fisher Scientific, catalog no. 09-719C). Nicotine doses are reported as free base. Recombinant HHIP protein was purchased from R&D systems (catalog no. 1568-HP). Murine Shh protein (catalog no. 315-22) and human Shh protein (catalog no. 100-45) were purchased from Preprotech and used at a final concentration of 3 µg⋅mL−1.
Caligiuri S.P., Howe W.M., Wills L., Smith A.C., Lei Y., Bali P., Heyer M.P., Moen J.K., Ables J.L., Elayouby K.S., Williams M., Fillinger C., Oketokoun Z., Lehmann V.E., DiFeliceantonio A.G., Johnson P.M., Beaumont K., Sebra R.P., Ibanez-Tallon I, & Kenny P.J. (2022). Hedgehog-interacting protein acts in the habenula to regulate nicotine intake. Proceedings of the National Academy of Sciences of the United States of America, 119(46), e2209870119.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!