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Unifi software v 1.8.2

Manufactured by Waters Corporation
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Sourced in United Kingdom

UNIFI software v. 1.8.2. is a data processing and analysis platform developed by Waters Corporation. It provides a standardized workflow for the acquisition, processing, and reporting of mass spectrometry data.

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Lab products found in correlation

Vion ims qtof, by Waters Corporation (2 mentions) Acquity uhplc 1 class system, by Waters Corporation (1 mentions)

Spelling variants of this product

UNIFI software (59 citations) UNIFI 1.8 (50 citations) UNIFI (23 citations)

2 protocols using unifi software v 1.8.2

1

Quantitative Proteomics by LC-MS/MS

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The LC-MS/MS system was comprised of a Waters Acquity H-Class Bio UHPLC System with an MS/MS detector. A Waters Vion IMS Qtof instrument was operated in positive ion/sensitivity mode at an m/z range of 400–4,000. The capillary voltage was set at 2.75 kV and the cone voltage at 140 V with a source temperature of 150 ºC and a desolvation temperature of 600 ºC. Instrument control, data processing, and deconvolution were performed using the Waters UNIFI software v. 1.8.2. with an advanced maximum entropy (MaxEnt) based procedure. The samples were analyzed on a Waters ACQUITY UPLC BEH C4 column (300 Å, 1.7 μm, 2.1 × 50 mm) at 80 ºC with a gradient of 0.1 % formic acid in water and 0.1 % formic acid in acetonitrile (5–50 %, 0.1 % formic acid in acetonitrile for 5 min).
Ashida H., Fujimoto T., Kurihara S., Nakamura M., Komeno M., Huang Y., Katayama T., Kinoshita T, & Takegawa K. (2020). 1,6-α-L-Fucosidases from Bifidobacterium longum subsp. infantis ATCC 15697 Involved in the Degradation of Core-fucosylated N -Glycan. Journal of Applied Glycoscience, 67(1), 23-29.
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2

UHPLC-IMS-HRMS Metabolomics Analysis

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Ultra-high performance liquid chromatography (UHPLC) with a ACQUITY UHPLC I-Class system (Waters, Milford, MA, USA) was coupled to a VION® IMS QTof (Waters, Manchester, UK), ion mobility hybrid Quadrupole Time-of-Flight (IMS-QTOF) High Resolution Mass Spectrometer (UHPLC-IMS-HRMS) using a electrospray ionization interface operating in both positive (ESI+) and negative (ESI−) mode. Equipment control and data acquisition and processing were performed using UNIFI software (V.1.8.2, Waters, Manchester, UK).
Lacalle-Bergeron L., Portolés T., López F.J., Sancho J.V., Ortega-Azorín C., Asensio E.M., Coltell O, & Corella D. (2020). Ultra-Performance Liquid Chromatography-Ion Mobility Separation-Quadruple Time-of-Flight MS (UHPLC-IMS-QTOF MS) Metabolomics for Short-Term Biomarker Discovery of Orange Intake: A Randomized, Controlled Crossover Study. Nutrients, 12(7), 1916.
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