Anti mir 29b 3p

The human cardiac fibroblasts (HCFs) were provided by ScienCell Research Laboratory (San Diego, CA). All cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; Invitrogen Life Technologies, Carlsbad, CA, USA) and penicillin–streptomycin solution in an incubator with 5% CO₂ and 95% air at 37°C. The HAFs were treated with 10⁻⁷ mol/L angiotensin II (AngII; Sigma-Aldrich, St. Louis, MO, USA) for 24 h to establish a cell model of AF as previously reported (19 (link)).
To regulate the gene expression level, cells were transfected with TUG1 siRNA (si-TUG1) or its negative control (si-NC) and with miR-29b-3p inhibitor (anti-miR-29b-3p) or its negative control (anti-NC) before AngII treatment, which were purchased from RiboBio (Guangzhou, China). In brief, si-TUG1, si-NC, anti-miR-29b-3p, or anti-NC (50 nmol/L) was transfected into logarithmic growth HAFs by using Lipofectamine 2000 and incubated in DMEM for 24 h before AngII treatment.

LNCaP cells with 30-40% confluence were incubated with antibiotic free RPMI-1640 medium for 24 h at 37 °C. Transfection was performed using the Trans IT®-2020 transfection reagent (Mirus Bio LLC) according to manufacturer's protocol. The sequences of the miR-29b-3p mimics (miR-29b-3p), inhibitors (anti-miR-29b-3p) and corresponding negative control (miR-con or anti-con) (RIBO Bio., Guangzhou) were presented in Table S3. MiR-29b-3p mimics and inhibitors were transfected in LNCaP cells at a final concentration of 50 and 100 nM, respectively.