Ha tag

Antibodies from Affinity Biosciences: p-Smad1/5/8 (1:1000, #AF8313), Smad1/5/8 (1:1000, #AF0614), PPARγ (1:1000, #bs-4590R), UCP1 (1:1000, #72298). Antibodies from Beyotime: HA tag (1:1000, #AH158), Flag tag (1:1000, #AF519). Antibodies from Sangon Biotech: p-ACC (1:1000, #D155180), ACC (1:1000, #D155300). Antibodies from Abcam: BMP8A (1:1000, #154373). Antibodies from ZenBio: p-AMPK (1:1000, #R26252), AMPK (1:1000, #380431), PGC-1α (1:1000, #381615). Antibodies from CWBIO: goat anti-Rabbit IgG HRP secondary antibody (1:4000, #CW0103S), goat anti-Mouse IgG HRP Conjugated (1:4000, # CW0102). Antibodies from Bioss: p38 MAPK (1:1000, #bs-0637R), p-p38 MAPK (1:1000, #bs-2210R), β-actin (1:2000, #bs-0061R), p-JNK (1:1000, #bs-1640R), JNK (1:1000, #bs-2592R), C/EBPα (1:1000, #AF6333), p-Smad2/3 (1:1000, #AF3367), Smad2/3 (1:1000, #AF6367), p-ERK1/2 (1:1000, #AF1015), ERK1/2 (1:1000, #AF0155), p-p65 (1:1000, #AF2006), p65 (1:1000, #AF5006), p-IKKα/β (1:1000, #AF3013), IKKα/β (1:1000, # AF6014).

The firefly luciferase reporter plasmid for IFN-γ-responsive promoter and the Renilla luciferase control plasmid (pRL-TK) (31 (link)–33 (link)) were kindly provided by Dr. Hong-Bing Shu (Wuhan University, China). The NSs or NP expression plasmids were constructed as described previously (10 (link), 13 (link), 18 (link)). Mouse anti-NSs antiserum or rabbit antisera to NSs, NP, GP (N-terminal domain), or RdRp were respectively raised against the corresponding viral proteins generated by Escherichia coli (10 (link), 18 (link)). Rabbit antibodies to S-tag (Abcam), STAT1 (Cell Signaling Technology), or STAT2 (Santa Cruz Biotech) and mouse antibodies to HA-tag (Beyotime), β-actin (Beyotime), or STAT1 (Santa Cruz Biotech) were purchased from the indicated manufacturers. Secondary antibodies include goat anti-mouse IgG-fluorescein isothiocyanate (FITC) (Proteintech), goat anti-rabbit IgG-Rhodamine (Chemicon), and goat anti-rabbit IgG-Cy5 (Abcam). Recombinant mouse or human IFN-γ proteins were purchased from Cell Sciences or Peprotech Inc., respectively. Recombinant human IFN-α was from PBL Biomedical Laboratories.