The FinePure Universal DNA Purification Kit was purchased from GENFINE (Beijing, China). The CYP2J2 Human Tagged ORF Clone (RC207417) was purchased from Origene (United States). Saccharomyces cerevisiae strain YPH499 was obtained from ATCC (VA, United States). The yeast expression vector pESC-URA was purchased from Stratagene (United States). The following primary antibodies were used: rabbit polyclonal anti-CYP2J2 antibody (Abcam, United Kingdom), mouse monoclonal anti-OR antibody (Santa Cruz Biotechnology, United States) and corresponding secondary antibodies (Proteintech, China). Ebastine and terfenadine were purchased from Tokyo Chemical Industry. HydroxyEbastine, terfenadine alcohol and midazolam were purchased from Santa Cruz and TRC. The NADPH-regenerating system was purchased from Promega (United States). High-pressure liquid chromatography-grade solvents were purchased from Fisher Scientific Co., (United States). All the other chemicals and solvents were commercially available at analytical grade or the highest grade.
Corresponding secondary antibodies
Manufactured by Proteintech
Sourced in United States
Corresponding secondary antibodies are laboratory reagents used to detect and amplify the signal of primary antibodies in various immunoassays, such as Western blotting, immunohistochemistry, and ELISA. These secondary antibodies are specifically designed to bind to the Fc region of primary antibodies, enabling the visualization and quantification of target proteins or molecules.
Automatically generated - may contain errors
Lab products found in correlation
Nadph regenerating system, by Promega (1 mentions)
Mouse monoclonal anti or antibody, by Santa Cruz Biotechnology (1 mentions)
High pressure liquid chromatography grade solvents, by Thermo Fisher Scientific (1 mentions)
Ebastine, by Tokyo Chemical Industry (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
2 protocols using corresponding secondary antibodies
1
Enzymatic Activity of CYP2J2 in Yeast
2
Protein Expression Analysis in Renal Tissues
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Renal cortical and outer medulla tissues were lysed, separated by 10% SDS-PAGE gels, and then transferred onto PVDF membranes. The membranes were blocked with 3% nonfat dried milk + 2% BSA in Tris-buffered saline Tween (TBST) for 2 hr at room temperature, and subsequently incubated with primary antibodies against GRP78 (1:1000, Cell Signaling Technology, 3177s), CHOP (1:1000, Santa-Cruz Biotechnology, sc-7351), Cleaved Caspase-3 (1:500, Cell Signaling Technology, 9661s), GAPDH (1:4000, Cell Signaling Technology) and corresponding secondary antibodies (1:2000, Proteintech Group, Chicago, IL, USA) respectively. The immunoreactive bands were detected by ECL and visualized on Kodak X-ray films. Each band was analyzed using an Image J image analysis system.
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