Epiquik dna methyltransferases activity inhibition assay kit

DNMT activity in amygdala and BNST tissues was measured using the EpiQuik^™ DNA methyltransferases activity/inhibition assay kit (Epigentek; Brooklyn, NY), as described by us previously (Zhang et al., 2013 (link)). Nuclear fractions of the tissue lysates were prepared using a nuclear extraction kit (Sigma, St. Louis, MO). 10 μg of nuclear protein was used for measuring the DNMT activity according to the manufacturer’s protocol and activity was assayed in terms of optical density (O.D.) using an ELISA plate reader at 450 nm (Dynex Technologies, Chantilly, VA). The results were calculated as O.D. /mg protein and are represented as the mean ± SEM.

DNMT activity in amygdala was measured using the EpiQuik™ DNA methyltransferases activity/inhibition assay kit (Epigentek; Brooklyn, NY), as described previously (Sakharkar et al., 2014a (link)). Nuclear protein fraction was prepared from the amygdaloid tissues using a nuclear extraction kit (Sigma, St. Louis, MO) and 10 µg of nuclear protein was used for measuring the DNMT activity according to the manufacturer’s protocol. Activity was measured in terms of optical density (O.D.)/mg protein and the results are presented as mean percent of controls (± SEMs).