Citral cit

Ethylene-vinyl alcohol copolymer with 29% ethylene molar content (EVOH-29) was supplied by The Nippon Synthetic Chemical Industry Co., Ltd. (Osaka, Japan). Trans-cinnamaldehyde (3phenyl-2-propenal) (CINHO), linalool (LIN), isoeugenol (IEG) and citral (CIT) were supplied by Sigma-Aldrich (Barcelona, Spain) . The films were made as described by Mateo et al. (2017a) . Briefly, films of EVOH with an initial content of 1, 2, 5 and 10% of EOC were prepared by casting in an oven at 75 °C for 15 min. The final content of EOC was determined by thermal desorption-gas chromatography as described by Cerisuelo et al. (2012) . The response of the gas chromatograph was calibrated by measuring polyethylene and polypropylene samples with known amounts of CINHO, CIT, IEG and LIN. The additive content is expressed as weight percentage of the compound over dry polymer weight. All the obtained EVOH-29 films containing pure components of plant essential oils were colorless, transparent and continuous. The final contents for CINHO, CIT, IEG or LIN found in the films, were (0.37 ± 0.06)%, (0.74 ± 0.12)%, (1.85 ± 0.25)% and (3.7 ± 0.5)%, w/w of dry polymer, for the films prepared at 1, 2, 5 and 10%, respectively, regardless of the EOC type. The grammage of the bioactive EVOH films was (1.79 ± 0.02) mg/cm 2 . The obtained films were labelled as EVOH-CINHO, EVOH-CIT, EVOH-IEG or EVOH-LIN.